Structure of PDB 2xct Chain U Binding Site BS03

Receptor Information

>2xct Chain U (length=665) Species: 158879 (Staphylococcus aureus subsp. aureus N315)

LADCSSKSPEECEIFLVEGDSAGGSTKSGRDSRTQAILPLRGKILNVEKA
RLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTDADVDGAHIR
TLLLTFFYRFMRPLIEAGYVYIAQPPTKGLGEMNADQLWETTMNPEHRAL
LQVKLEDAIEADQTFEMLMGDVVENRRQFIEDNAVYNITSEMRESFLDYA
MSVIVARALPDVRDGLKPVHRRILYGLNEQGMTPDKSYKKSARIVGDVMG
KYHPHGDSSIYEAMVRMAQDFSYRYPLVDGQGNFGSMDGDGAAAMRFTEA
RMTKITLELLRDINKDTIDFIDNYDGNEREPSVLPARFPNLLANGASGIA
VGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDFPTAGLILG
KSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTP
LQTSFGVNMIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKD
RAHILEGLRIALDHIDEIISTIRESDTDKVAMESLQQRFKLSEKQAQAIL
DMRLRRLTGLERDKIEAEYNELLNYISELETILADEEVLLQLVRDELTEI
RDRFGDDRRTEIQLG

Ligand information

>2xct Chain X (length=11)

gtacccacggc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2xct Type Iia Topoisomerase Inhibition by a New Class of Antibacterial Agents.
• Resolution: 3.35 Å
• Binding residue (original residue number in PDB): D437 S438 R1122 I1175 V1177 G1178 M1179 R1238
• Binding residue (residue number reindexed from 1): D20 S21 R296 I349 V351 G352 M353 R412

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

External links

• PDB: RCSB:2xct, PDBe:2xct, PDBj:2xct
• PDBsum: 2xct
• PubMed: 20686482
• UniProt: P66937|GYRB_STAAN DNA gyrase subunit B (Gene Name=gyrB);
  Q99XG5|GYRA_STAAN DNA gyrase subunit A (Gene Name=gyrA)