Structure of PDB 8fld Chain SI Binding Site BS03

Receptor Information

>8fld Chain SI (length=234) Species: 9606 (Homo sapiens)

PLVPENLLKKRKAYQALKATQAKQALLAKKEQKKGRFKRLESFLHDSWRQ
KRDKVRLRRLEVKPHALELPDKHSLAFVVRIERIDGVSLLVQRTIARLRL
KKIFSGVFVKVTPQNLKMLRIVEPYVTWGFPNLKSVRELILKRGQAKVKN
KTIPLTDNTVIEEHLGKFGVICLEDLIHEIAFPGKHFQEISWFLCPFHLS
VARHATKNRVGFLKEMGTPGYRGERINQLIRQLN

Ligand information

>8fld Chain L3 (length=3372)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccggggcgcgggacauguggc
guacggaagacccgcuccccggcgccgcgggggcccaaguccuucugauc
gaggcccagcccguggacggugugaggccgguagcggcccccggcgcgcc
gggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcg
ggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaa
caaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaaga
gggcgugaaaccguuaagaggaacgggugggguccgcgcaguccgcccgg
aggauucaacccggcggcggccggccgugucggcggcccggcggaucugg
gaccgucccccgaccggcgaccggccgccgccgggcgcauuuccaccgcg
gcggugcgccgcgaccggcuccgggacggcugggaaggcccggcggggaa
gguggcucgggcccgaguguuacagcccccccggcagcagcacucgccga
aucccggggccgagggaggucgccgcgcucuccccccggggggccgggcc
accccucccacggcgcgaccgcucggggcggacuguccccagugcgcccc
gggcgggucgcgccgucgggcccggggggccacgcgcgcgucccccgaag
agggggacggcggagcgagcgcacggggucggcggcgacgucggcuaccc
acccgacccgucuugaaacacggaccaaggagucuaacacgugcgcgagu
cgggggcucgcacgaaagccgccguggcgcaaugaaggugaaggccgcgg
ccgaggugggaucccgaggccucuccaguccgccgagggcgcaccaccgg
cccgucucgcccgccgcgccggggagguggagcacgagcgcacguguuag
gacccgaaagauggugaacuaugccugggcagggcgaagccagaggaaac
ucugguggagguccguagcgguccugacgugcaaaucggucguccgaccu
ggguauaggggcgaaagacuaaucgaaccaucuaguagcugguucccucc
gaaguuucccucaggauagcuggcgcucuccgcaguuuuauccgguaaag
cgaaugauuagagguaccuauucucaaacuuuaaauggguaagaagcccg
gcucgcuggcguggagccgggcguggaaugcgagugccuagugggccacu
uuugcagaacuggcgcugcgggaugaaccgaacgccggguuaaggcgccc
gaugccgacgcucaucagaccccaaaagguguugguugauauagacagca
ggacgguccgcuaagaccugccgaaucaacuagcccugaaaauggauggc
gcuggagcgucgggcccauacccggccgucgccggcagucgagaguggac
gggagcggcgcgcggacgcuacgccgcgacgaguaggagggccgcugcgg
ugagccuugaagccuagggcgcgggcccggguggagccgccgcaggugca
gaucuuggugguaguagcaaauauucaaacgagaacuuugaaggccgaag
uggagaaggguuccaugugaacagcaguugaacaugggucagucgguccu
gagagaugggcgagcgccguuccgaagggacgggcgauggccuccggccc
ugaucgaaagggagucggguucagauccccgaauccggaguggcggagau
gggcgccgcgaggcguccagugcgguaacgcgaccgaucccggagaagcc
ggcgggagccccggggagaguucucuuuucuuugugaagggcagggcgcc
cuggaauggguucgccccgagagaggggcccgugccuuggaaagcgucgc
gguuccggcggcguccggugagcucucgcuggcccuugaaaauccggggg
agaggguguaaaucucgcgccgggccguacccauauccgcagcaggucuc
caaggugaacagccucuggcauguuggaacaauguagguaagggaagucg
gcaagccggauccguaacuucgggauaaggauuggcucuaagggcugggu
cggucgggcuggggcgcgaagcggggucccguggaucgccccagccggcc
ggcgccuagcagccgacuuagaacuggugcggaccaggggaauccgacug
uuuaauuaaaacaaagcaucgcgaaggcccgcggcggguguugacgcgau
gugauuucugcccagugcucugaaugucaaagugaagaaauucaaugaag
cgcggguaaacggcaagugaacgagauucccacugucccuaccuacuauc
cagcgaaaccacagccaaggcgggcuuggcggaaucagcggggaaagaag
acccuguugagcuugacucuagucuggcacggugaaguuuuuucacugac
ccggugaggcggggggcuucuggcgccaagcgcccggccgcgcgccggcc
gggcgcgacccgcuccggggacagugccagguggggaguuugacuguaca
ccugucaaacgguaacgcagguguccuaaggcgagcucagggaggacaga
aaccucccguggagcagaagggcaaaagcucgcuugaucuugauuuucag
uacgaauacagaccgugaaagcggggccucacgauccuucugaccuuuug
gguuuuaagcaggaggugucagaaaaguuaccacagggaugaugucggcu
cuuccuaucauugugaagcagaauucaccaagcguuggauuguucaccca
cuaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuu
uacccuacugaugauguguuguugccaugguaauccugcucaguacgaga
ggaaccgcagguucagacauuugguguaugugcuuggcugaggagccaau
ggggcgaagcuaccaucugugggauuaugacugaacgccucuaagucaga
aucccgcccaggcggaacgauagcgccgcggagccucgguuggccucgga
uagccgguccccgggguccggugcggagugcccuucguccugggaaacgg
ggcgcggccggagaggcggccgcccccucgcccgucacgcaccgcacguu
cguggggaaccuggcgcuaaaccauucguagacgaccugcuucugggucg
ggguuucguacguagcagagcagcucccucgcugcgaucuauugaaaguc
agcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
>>>>>.>.........<<....>>..<<<<<<.....<<.....>>....
>>>>>>..<<<..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>
>>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......
>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>
>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....<
<<<<..((((((>>>>>..>>>............................
......<<....>>...............<<<<....>>>>.........
.......................<<<<<<<<..<<<.<<...<<<<<<<<
.<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<...
>>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>.>
>>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<..
....<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>>.
.>>>>>>>>>>>............<<<<<<.<<<<<..>>>>>.<<<<<<
.<<<<.<<<<<<<<<<..<<<<<<<<<<<<<...........>.>>>>>>
>>>>>>...>>>>>>>.>>>...>>>>>>>>.>>..<<<<<<<<<.....
..>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>>>...>>>
>>>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..<<
<...<<<<........>>>>.....>>>...<<....>>...<<<<..>>
>>..<<<<<...<<<..<<<..((((.((((>>>..>>>....>>>>>.<
<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>
..<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>
>>>>>>.....>>>...<<<..<<.<<........>>>>.>>>....>>>
>>>>>>...<<.......>>......>>>>>>...>..>.>>..<<<<<<
....<.<<<....>>>.><<.<<<<<<<...<<<.<<<<<<<<<...<<<
....<<<..<.<<<..>>>.>..>>>..>>.>...>>>>>>>...<<<<<
<<<<.........>>>>>>>>>....>>>>>>>>>>>>..>>..<<<<.<
<<<..>>>>.>>>>....<<<......>>>...<<<<<<<...<<<(((.
......<<<<<.....<<........<<<.<<<<<<<<<<.......<<<
<<.<<<.>>>......>>>>>...>>>>>>>>>>>>>..........)))
.>>...>>>>>..>>>...>>>>>>><<<<<<.<<<..........))))
.)))).................>>>>>>>>>....>>>>>>.<.<<<<<<
<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>..
...>>>>>><<.....<<.....<<<<<<.......>>>>>>.<<<....
.<<<<<.<<...<<<<<....<<.....>>..>>>>>....((<<<<<<.
..<<....<<<....>>>.............<<<<...........>>>>
...>>....>>>..<<<<<........>>>>>))<<<<....<<<<<..<
<<<<<<<....>>>>>>>>..<<<....>>>...<..<<<<<<<...<<<
<<<<<<<<<<<<<<<<<<<<..[[[..>>>>>>....<<<<<.<<<<.<<
<<.....<<<.....>>>.....>>>>>>>>..>>>>>.....<<<<<<<
.......>>>>>>>>>>>.>.>>>>>>>>>>>>.........>>>>>>>.
.>..........>>.>>>.>>>>.>>>..>>.....<<......>>>>>>
>.<<<<.....>>>>..>>>....<<<<.....<<<<<<<.<<.......
........<<<<<....<<..>>.....>>>>><<.<<<<<<<<<<<.<<
<<<<<<.<<<<<<<<<....<<<<<..>>>>>....>>>>>>>>>>>>>>
>>>......>>>>>..>>>>>>.>>............<<<<<<<<<..<<
<.........>>>..<<<<<<<....<<<<.....>>>>......>>>>>
>>.......<<<..<<<<<<.<..<<<.....<<<<....>>>>.....>
>>>>>>>..>>>>>.......>>.>>>>>>>.....>>.>>>>>>>..>>
>>>>....>>...<<<<<<.....>>>>>>...<<<<<..<<<.......
.<<<<<.<<<<<<..<<<<<.<<<<<<<<<.<<<<<.....>>>>>...<
<<<<.....<<<<................<<<<<<<<<<.......>>>>
>>>>>>..>>>>..>>>>>....>>>>>>>>>..>>>>>...<<.>>..<
<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<.....
..>>>>>>.....<<.[.(((>>....>>>>>>>...<<<..<<<<....
...>>>>..>>>.<<<....>>>.>>)))].....<<<<<<<..<<<..)
))))).>>>>>>>>>>.........>>>>..>>>>>>>..((...<<<<.
<<<<<<..<.<.<<<<<......>>>>>...>.>.<<<.<...))>.>>>
.....>>>>>..>...>>>>......<<<<<<<....>>>.>>>>.....
...>>>.>>>>>.....<<<<<<.<..<<<..<.<<<<<...........
......>>>>>.><<<<....<<<<....<<.<<<<<<.]]]..>>>>..
>>.>>...>>>>...>>>><<<<<...<<<<<...........>>>>>..
.>>>>>.>>>...>.>>>>>>.<<<<<<.<<..<<<<....<<<......
..<<<<<.<<<<>>>>.>>>>>.......>>>...<<<..<<<<....<<
<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>...........
...>>>>..>>>>>>>>.<<<<<.<.............>....<<.<<<<
<<<.<<.....<<<<....<<<<<......>>>>>...>>>>....>>..
..>>>.>>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fld Principles of human pre-60S biogenesis
• Resolution: 2.58 Å
• Binding residue (original residue number in PDB): Q39 K47 K123 A223 R224 H225
• Binding residue (residue number reindexed from 1): Q21 K29 K102 A202 R203 H204

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0001825 blastocyst formation

Cellular Component

• GO:0005730 nucleolus
• GO:0005739 mitochondrion
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fld, PDBe:8fld, PDBj:8fld
• PDBsum: 8fld
• PubMed: 37410842
• UniProt: Q6DKI1|RL7L_HUMAN Ribosomal protein uL30-like (Gene Name=RPL7L1)