Structure of PDB 8fkw Chain SE Binding Site BS03

Receptor Information

>8fkw Chain SE (length=185) Species: 9606 (Homo sapiens)

KWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPE
TKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLV
VIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFT
QVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGN

Ligand information

>8fkw Chain L3 (length=2277)

gcgaccucagaucagacguggcgacccgcugaaagcauauuagucagcgg
aggagaagaaacuaaccaggauucccucaguaacggcgagugaacaggga
agagcccagcgccgaauccccgccccgggggcgcgggacauguggcguac
ggaagacccgcuccccggcgccgcucguggggggcccaaguccuucugau
cgaggcccagcccguggacggugugaggccgguagcggcccccggcgcgc
cgggcccgggucuucccggagucggguugcuugggaaugcagcccaaagc
gggugguccaucuaaggcuaaauaccggcacgagaccgauagucaacaag
uaccguaagggaaaguugaaaagaacuuugaagagagaguucaagagggc
gugaaaccguuaagaggaacgggugggguccgcgcaguccgcccggagga
uucaacccggcggcggccgccgccgggcgcauuuccaccgcggcggugcg
ccgcgaccggcuccgggacggcugggaaggcccggcggggaagguggcuc
gggcccgaguguuacagcccccccggcagcagcacucgccgaaucccggg
gccgagggagcgccgcgcucuccggccgggccaggccacggagcgcacgg
ggucggcggcgacgucggcuacccacccgacccgucuugaaacacggacc
aaggagucuaacacgugcgcgagucgggggcucgcacgaaagccgccgug
gcgcaaggugggaucccgaggcccgccgagggcgcaccaccggcccgucu
cgcccggggagguggagcacgagcgcacguguuaggacccgaaagauggu
gaaaccaucuaguagcugguucccuccgaaguuucccggauagcuggcgc
ucuccgcaguuuuauccgguggguaagaagcccggcucgagccgggcgug
gaaugcgagugccuagugggccacuuuugguaagcagaacuggcgcugcg
ggaugaaccgaacgccggguuaaggcgcccgaugccgacgcucaucagac
cccagaaaagguguugguugauauagacagcaggacgguggccauggaag
ucggaauccgcuaaggaguguguaacaacucaccugccgaaucaacuagc
ccugaaaauggauggcgcuggagcgucgggcccauacccggccgucgccg
gcagucacgcuacgccgcgacgaguaggagggccgcugcggugagccuug
aagccuagggcgcgggcccggguggagccgccgcaggugcagaucuuggu
gguaguagcauucaaacgagaacuuugaaggccgaaguggagaaggggcg
auggccuccggcccucgcggagaugggcgccgcgaggcguccagugaauc
ucgcccgcagcaggucuccggugaacagccucuggcaauguagguaaggg
aagucgggaauccgauucccacugucccuaccuacuauccagcgaaacca
cagccaagggaacgggcuuggcggaaucagcggggagaagacccuguuga
gcuugacucuagucuggcacggugaagagacaugagagguguagaauaag
ugggaggcggccgccggugaaauaccacuacucugaucguuuuuuccagu
gccagguggggaguuauccuucugaccuuuuggguuuuaagcaggaggug
ucagaaaaguuaccacagggauaacuggcuuguggcggccaagcgcgacg
ucgcuuuuugaugaugucggcucuuccuaucauugugaagcagaauucac
caagcguuggauuguucacccacuaauagggaacgugagcuggggaccgu
cgugagacagguucccuacugaugguuguugccaugguaauccugcucag
uacgagaggaaccgcagguucagacauuugguggugcuuggcugaggagc
caauggggcgaagcuaccaucugugggauuaugacugaacgccucuaagu
cagaaucccgcccaggcggaacgauaagcgccgcggagccucgguuggcc
ucggauagccgguccccgggguccggugcggagugcccuucguccuggga
aacggggcgcggccggagaggcggccgcccccucgcccgucacgcaccgc
acguucguggggaaccuggcgcuaaaccauucguagacgaccugcuucug
ggucgggguuucguacguagcagagcagcucccucgcugcgaucuauuga
aagucagcccucgacacaaggguuugu

........................<<<<<<...............>>>>>
.>.........<<....>>..<<<<<<.....<<.....>>....>>>>>
>..<<<..<<<<<...<<<<<<<<<<.>>>>>>.>>>>.....>>>>>..
<<<<<<<<<<...<<<<<<<<.<<.....<<<<<<<<....<<<<.....
.>>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>
>>>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....
<<<<<<.>>>>>>..>>>................................
..<<....>>...............<<<<....>>>>.............
...................<<<<<<<<.<<<<.<<...<<<<<<<<.<<<
.....<<<<<<<<<<..>>>>>>>>>>......>>>.>>>.>>>>>...<
<<<<<<.<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<......<<<<
<<..>>>>>>.......>>>>>>.>>>........>>>>>>..>>>>>>>
>>>>..........<<<<<<.<..>.<<<<<<.>>>>.>>>>>>>>....
..>>.>>>>>...>>>>>..>>>>>>>>>..<<<<.<....>.>>>><<<
<<<....<<<<<<<<<<<<<..<<<...<<<<........>>>>.....>
>>....<<<<<...<<<..<<<..>>>..>>>....>>>>>.<<<<<<<<
<.<<..>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<...<<<<<<
...>>>>>>......>.>>..<<<<<<......<<<.>>>..<<.<<<<<
<<...<<<.<<<<<<<<...>>>>>>...<<<<<<<<..>>>>>>>>...
.>>>>>>>>>>>>..>>..<<<<.<<<<<.....>>>>>.>>>>....<<
<......>>>...<<<<<<<...<<<(((.......<<<<<.....<<..
........<<<.<<<<<<<<<<.....<.<<<<<.<<<...<<.......
..>>...>>>.........<<......>>...>>>>>.>.>>>>>>>>>>
>>>..........))).>>...>>>>>..>>>...>>>>>>><<<<<<.<
<<...........>>>>>>>>>....>>>>>>.<.<<<<<<<<.<<....
..<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..>.....>>>>>>
<<.....<<.<<<<<<.......>>>>>>.<<<<....<<<<...<<<<.
..........>>>>..<<<<<...<<<<<<<....>>>>>>>......>>
.>>><<......>>.>>>><<<.....>>>..>>>>...<<<<<<.<<<<
<<<<..<<<<<<.>>>>>>.>>>.>>>>>>>>>>>......>>....>>.
..<<<<<<........>>>>>>....<<<<.<<<<......<<<<<<.<<
<<<<.<<<<<.<<<<<<<<<...<<<<<<<<<<.<<<<..........<<
<<<..<<<.>>>............>>>>>.>>>>.>>.>>>>>>>>..>>
>>>>>>>..>>>>>...<<<<<<<..<<<.........>>>>>>>>>>..
......>>>>>.>.>>>>>>.............<<<<<<...<..>...>
>>>>>.......((...<<<<<<<<<<<..<.<.<<<<<......>>>>>
...>.>.<<<.....))..>>>.....>>>>>....>>>>>>..<<<<<<
<....>>>.>>>>>>>>.>>>>..<<<<<<.<..<<<..<.<<<<<....
.............>>>>>.><<<<....<<<<.<<.<<<<<<<....>>>
>>..>>.>>...>>>>...>>>><<<<<...<<<<<...........>>>
>>...>>>>>.>>>...>.>>>>>>.<<<<<<<.<<..<<<<....<<<.
.......<<<<<.<<<<>>>>.>>>>>.......>>>...<<<..<<<<.
...<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>......
........>>>>..>>>>>>>>><<<<<.<.............>....<<
.<<<<<<<.<<.....<<<<....<<<<<......>>>>>...>>>>...
.>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fkw Principles of human pre-60 S biogenesis.
• Resolution: 2.5 Å
• Binding residue (original residue number in PDB): P58 R59 Y60 K110 K111 Q112 R132 P133 V135 R137 G139 N141 T142 T145 D162 L166 A188 R196 K197 T198 C199 T200
• Binding residue (residue number reindexed from 1): P3 R4 Y5 K55 K56 Q57 R77 P78 V80 R82 G84 N86 T87 T90 D107 L111 A133 R141 K142 T143 C144 T145

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0045296 cadherin binding

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fkw, PDBe:8fkw, PDBj:8fkw
• PDBsum: 8fkw
• PubMed: 37410842
• UniProt: P62424|RL7A_HUMAN Large ribosomal subunit protein eL8 (Gene Name=RPL7A)