Structure of PDB 8pfg Chain J Binding Site BS03

Receptor Information
>8pfg Chain J (length=1317) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCARIFGPV
KDTQTKVRRERMGHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLY
FESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKMGAEAIQALLK
SMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDI
IVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQ
NLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGL
ATTIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPV
LIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNI
LSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSG
LASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYS
IVNQALGKKAISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVG
IDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKVIDIWAAAN
DRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKT
ANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVL
GRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDT
DFGVCAHCYGRDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFESSIQ
VKNKGSIKLSNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGA
VLAKGDGEQVAGGETVANWDPHTMPVITEVSGFVRFTDMIDGQTITRQTD
ELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDVLIPGTDMPAQYFL
PGKAIVQLEDGVQISSGDTLARIPQITGGLPRVADLFEARRPKEPAILAE
ISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERVERGD
VISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVR
QMLRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLG
ITKASLATESFISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIP
AGTGYAYHQDRMRRRAA
Ligand information
>8pfg Chain A (length=40) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
caccaccacgcgggcggtagcgtgcttttttcgatcttcc
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8pfg Concerted transformation of a hyper-paused transcription complex and its reinforcing protein.
Resolution3.1 Å
Binding residue
(original residue number in PDB)		R133 R271 R278 R314 R1148 K1170
Binding residue
(residue number reindexed from 1)		R93 R231 R238 R274 R1090 K1112
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006352 DNA-templated transcription initiation
GO:0006879 intracellular iron ion homeostasis
GO:0009408 response to heat
GO:0031564 transcription antitermination
GO:0032784 regulation of DNA-templated transcription elongation
GO:0036460 cellular response to cell envelope stress
GO:0042128 nitrate assimilation
GO:0044780 bacterial-type flagellum assembly
GO:0046677 response to antibiotic
GO:0048870 cell motility
GO:0071973 bacterial-type flagellum-dependent cell motility
GO:0090605 submerged biofilm formation
GO:2000142 regulation of DNA-templated transcription initiation
Cellular Component
GO:0000345 cytosolic DNA-directed RNA polymerase complex
GO:0000428 DNA-directed RNA polymerase complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008023 transcription elongation factor complex
GO:0016020 membrane

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Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8pfg, PDBe:8pfg, PDBj:8pfg
PDBsum8pfg
PubMed38589445
UniProtP0A8T7|RPOC_ECOLI DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)

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