Structure of PDB 8fak Chain H Binding Site BS03

Receptor Information
>8fak Chain H (length=643) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
PVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGIVVSVSD
ASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALP
ILLRQGRPAANTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLE
NVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAW
LKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWRYHARD
LAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQ
HVLDLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDC
GWIAECPRCDHYYTLHQAQHHLRCHHCDSQRPVPRQCPSCGSTHLVPVGL
GTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGT
QMLAKGHHFPDVTLVALLDVDGALFSADFRSAERFAQLYTQVAGRAGRAG
KQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIV
RAEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPKRGGRWRW
QILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8fak Replication fork binding triggers structural changes in the PriA helicase that govern DNA replication restart in E. coli.
Resolution3.22 Å
Binding residue
(original residue number in PDB)		D17 Y18 F36 G37 L55 L60 K61 R719
Binding residue
(residue number reindexed from 1)		D16 Y17 F35 G36 L54 L59 K60 R630
Enzymatic activity
Enzyme Commision number 3.6.4.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006269 DNA replication, synthesis of primer
GO:0006270 DNA replication initiation
GO:0006276 plasmid maintenance
GO:0006302 double-strand break repair
GO:0006310 DNA recombination
GO:0010332 response to gamma radiation
GO:0031297 replication fork processing
GO:0032508 DNA duplex unwinding
GO:0046677 response to antibiotic
Cellular Component
GO:1990077 primosome complex
GO:1990158 DnaB-DnaC-DnaT-PriA-PriB complex
GO:1990159 DnaB-DnaC-DnaT-PriA-PriC complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8fak, PDBe:8fak, PDBj:8fak
PDBsum8fak
PubMed37169801
UniProtP17888|PRIA_ECOLI Primosomal protein N' (Gene Name=priA)

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