Structure of PDB 6ut6 Chain D Binding Site BS03

Receptor Information

>6ut6 Chain D (length=292) Species: 83333 (Escherichia coli K-12)

TESYCLEDALNDLFIPETTIETILKRLTIKKNIILQGPPGVGKTFVARRL
AYLLTGEKAPQRVNMVQFHQSYSYEDFIQGYRPNGVGFRRKDGIFYNFCQ
QAKEQPEKKYIFIIDEINRANLSKVFGEVMMLMEHDKRGENWSVPLTYSE
NDEERFYVPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFDTPQF
RNFLLNKKAEPSFVESLCQKMNELNQEISKEATILGKGFRIGHSYFCCGL
EDGTSPDTQWLNEIVMTDIAPLLEEYFFDDPYKQQKWTNKLL

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 6ut6 Chain D Residue 502

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6ut6 Structural asymmetry governs the assembly and GTPase activity of McrBC restriction complexes
• Resolution: 3.28 Å
• Binding residue (original residue number in PDB): T208 D279
• Binding residue (residue number reindexed from 1): T44 D115
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 3.1.21.-

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003924 GTPase activity
• GO:0004519 endonuclease activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0005525 GTP binding
• GO:0010385 double-stranded methylated DNA binding
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding
• GO:0044729 hemi-methylated DNA-binding

Biological Process

• GO:0009307 DNA restriction-modification system

Cellular Component

• GO:1905348 endonuclease complex

External links

• PDB: RCSB:6ut6, PDBe:6ut6, PDBj:6ut6
• PDBsum: 6ut6
• PubMed: 33219217
• UniProt: P15005|MCRB_ECOLI Type IV methyl-directed restriction enzyme EcoKMcrB subunit (Gene Name=mcrB)