Structure of PDB 8ej3 Chain C Binding Site BS03

Receptor Information

>8ej3 Chain C (length=1121) Species: 83332 (Mycobacterium tuberculosis H37Rv)

NSVPGAPNRVSFAKLREPLEVPGLLDVQTDSFEWLIGSPRWRESAAERGD
VNPVGGLEEVLYELSPIEDFSGSMSLSFSDPRFDDVKAPVDECKDKDMTY
AAPLFVTAEFINNNTGEIKSQTVFMGDFPMMTEKGTFIINGTERVVVSQL
VRSPGVYFDETIDKSTDKTLHSVKVIPSRGAWLEFDVDKRDTVGVRIDRK
RRQPVTVLLKALGWTSEQIVERFGFSEIMRSTLEKDNTVGTDEALLDIYR
KLRPGEPPTKESAQTLLENLFFKEKRYDLARVGRYKVNKKLGLHVGEPIT
SSTLTEEDVVATIEYLVRLHEGQTTMTVPGGVEVPVETDDIDHFGNRRLR
TVGELIQNQIRVGMSRMERVVRERMTTQDVEAITPQTLINIRPVVAAIKE
FFGTSQLSQFMDQNNPLSGLTHKRRLSALGPGGLSRERAGLEVRDVHPSH
YGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETPYRKVVDGVVSDEIV
YLTADEEDRHVVAQANSPIDADGRFVEPRVLVRRKAGEVEYVPSSEVDYM
DVSPRQMVSVATAMIPFLEHDDANRALMGANMQRQAVPLVRSEAPLVGTG
MELRAAIDAGDVVVAEESGVIEEVSADYITVMHDNGTRRTYRMRKFARSN
HGTCANQCPIVDAGDRVEAGQVIADGPCTDDGEMALGKNLLVAIMPWEGH
NYEDAIILSNRLVEEDVLTSIHIEEHEIDARDTKLGAEEITRDIPNISDE
VLADLDERGIVRIGAEVRDGDILVGKVTPKGREVRDTSLKVPHGESGKVI
GIRVFSREDEDELPAGVNELVRVYVAQKRKISDGDKLAGRHGNKGVIGKI
LPVEDMPFLADGTPVDIILNTHGVPRRMNIGQILETHLGWCAHSGWKVDA
AKGVPDWAARLPDELLEAQPNAIVSTPVFDGAQEAELQGLLSCTLPNRDG
DVLVDADGKAMLFDGRSGEPFPYPVTVGYMYIMKLHHLVDDKIHARSTGP
YSMITQQPLGGKAQFGGQRFGEMECWAMQAYGAAYTLQELLTIKSDDTVG
RVKVYEAIVKGENIPEPGIPESFKVLLKELQSLCLNVEVLSSDGAAIELR
EGEDEDLERAAANLGINLSRN

Ligand information

>8ej3 Chain T (length=32)

cggcagtcgccgtctacctctccaagagcagc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8ej3 Allosteric mechanism of transcription inhibition by NusG-dependent pausing of RNA polymerase.
• Resolution: 3.13 Å
• Binding residue (original residue number in PDB): R421 K1060 R1067
• Binding residue (residue number reindexed from 1): R392 K1012 R1019

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0046677 response to antibiotic

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:8ej3, PDBe:8ej3, PDBj:8ej3
• PDBsum: 8ej3
• PubMed: 36745813
• UniProt: P9WGY9|RPOB_MYCTU DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)