Structure of PDB 1flo Chain C Binding Site BS03

Receptor Information

>1flo Chain C (length=404) Species: 4932 (Saccharomyces cerevisiae)

PQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMITHNGTA
IKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWE
FTIIPYYGQKHQSDITDIVSSLQLQFESNSHSKKMLKALLSEGESIWEIT
EKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQN
KYLGVIIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKR
VNRTGNKQEYQLLKDNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMT
SFLSMKGLTELTNVVGNWSDKRASAVARTTYTHQITAIPDHYFALVSRYY
AYDPISKEMIALKDETNPIEEWQHIEQSIRYPAWNGIISQEVLDYLSSYI
NRRI

Ligand information

>1flo Chain J (length=19)

tttaaaagaataggaactt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1flo Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping.
• Resolution: 2.65 Å
• Binding residue (original residue number in PDB): H47 K53 A55 T56 Y60 K82 T83 Q84 F192 S193 K223 R281 K299 N300 K303 S304 H305
• Binding residue (residue number reindexed from 1): H46 K52 A54 T55 Y59 K81 T82 Q83 F184 S185 K215 R269 K287 N288 K291 S292 H293

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003690 double-stranded DNA binding
• GO:0003697 single-stranded DNA binding
• GO:0008301 DNA binding, bending
• GO:0009009 site-specific recombinase activity

Biological Process

• GO:0006310 DNA recombination
• GO:0015074 DNA integration
• GO:0042150 plasmid recombination

Cellular Component

• GO:0005575 cellular_component

External links

• PDB: RCSB:1flo, PDBe:1flo, PDBj:1flo
• PDBsum: 1flo
• PubMed: 11090626
• UniProt: P03870|FLP_YEAST Site-specific recombinase Flp (Gene Name=FLP1)