Structure of PDB 8a3v Chain B Binding Site BS03

Receptor Information

>8a3v Chain B (length=447) Species: 666 (Vibrio cholerae)

IPDAQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVSEHVMTQDFYSRPH
RLIFDGVKSILEAGKPLDLITLSEYLEQREQLEDVGGFAYLADLAKNTPS
AANINAYAEIVAERALVRNLIGVANEIADAGYDPQGRNAEDLLDLAESKV
FAIAEARTSENEGPKNVDSILERTLERIELLYKTPQDGVTGVNTGFTDLN
KKTAGLQGSDLIIVAARPSMGKTTFAMNLCENAAMEQDKPVLIFSLEMPA
EQIMMRMLASLSRVDQTKIRTGQLDDEDWARISSTMGILMEKKNMYIDDS
SGLTPTEVRSRARRIAREHGGLSLIMVDYLQLMRVPALTDNRTLEIAEIS
RSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLI
MFIYRDEVYHPDSPLKGTAEIIIGKQRNGPIGSVRLTFQGHYSRFDN

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 8a3v Chain B Residue 502

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8a3v The LH-DH module of bacterial replicative helicases is the common binding site for DciA and other helicase loaders.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): T235 D340 Y341
• Binding residue (residue number reindexed from 1): T223 D328 Y329
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0006268 DNA unwinding involved in DNA replication
• GO:0006269 DNA replication, synthesis of primer

Cellular Component

• GO:0005829 cytosol
• GO:1990077 primosome complex

External links

• PDB: RCSB:8a3v, PDBe:8a3v, PDBj:8a3v
• PDBsum: 8a3v
• PubMed: 36762863
• UniProt: A0A085R2T8