Structure of PDB 7z4c Chain B Binding Site BS03

Receptor Information
>7z4c Chain B (length=1357) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL
LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRL
EESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL
RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPI
NASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPN
FKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIF
FDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK
QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN
LPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDL
LFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKII
KDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQL
KRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDS
LTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVM
GRHKPENIVIEMAREKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNE
KLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTR
SDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLS
ELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLK
SKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRK
RPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKES
ILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKS
VKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENG
RKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFV
EQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENI
IHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETR
IDLSQLG
Ligand information
>7z4c Chain A (length=90) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aagaugagacgcguuuuagagcuagaaauagcaaguuaaaauaaggcuag
uccguuaucaacuugaaaaaguggcaccgagucggugcuu
............<<<<<<..<<<<....>>>>....>>>>>>..<<....
.>>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7z4c R-loop formation and conformational activation mechanisms of Cas9.
Resolution3.87 Å
Binding residue
(original residue number in PDB)		K3 K30 K33 L35 K44 N46 E57 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 K76 N77 L101 S104 F105 V107 R115 I122 E125 H129 T134 I135 H160 K163 F164 R165 G166 H167 Y325 H328 L332 K336 R340 F351 Y359 I363 Q402 R403 T404 F405 N407 G408 F446 I448 Y450 P454 L455 R457 N459 S460 F462 W464 T472 I473 P475 M495 N497 K500 G729 K735 Y981 M1089 P1090 E1099 V1100 T1102 G1103 G1104 F1105 I1110 R1122 K1123 K1124 Y1131 F1134 E1225 L1226 A1227 H1349 Q1350 S1351 Y1356
Binding residue
(residue number reindexed from 1)		K2 K29 K32 L34 K43 N45 E56 A58 T61 R62 K64 R65 R68 R69 R70 Y71 R73 K75 N76 L100 S103 F104 V106 R114 I121 E124 H128 T133 I134 H159 K162 F163 R164 G165 H166 Y324 H327 L331 K335 R339 F350 Y358 I362 Q401 R402 T403 F404 N406 G407 F445 I447 Y449 P453 L454 R456 N458 S459 F461 W463 T471 I472 P474 M494 N496 K499 G728 K734 Y972 M1080 P1081 E1090 V1091 T1093 G1094 G1095 F1096 I1101 R1113 K1114 K1115 Y1122 F1125 E1216 L1217 A1218 H1340 Q1341 S1342 Y1347
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:7z4c, PDBe:7z4c, PDBj:7z4c
PDBsum7z4c
PubMed36002571
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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