Structure of PDB 5aor Chain B Binding Site BS03

Receptor Information

>5aor Chain B (length=992) Species: 7227 (Drosophila melanogaster)

SVIDRIQEQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDDY
KYTPVGPEHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLVR
QLFHLNVIEPFSGTLKKKKDEQLKPYPVKLSPNLINKIDEVIKGLDLPVV
NPRNIKIELGPPIPLIVSVIPWAPPQANWNTWHACSIDDLSMDYERSLRD
RRQNDNEYRQFLEFREKLPIAAMRSEILTAINDNPVVIIRGNTGCGKTTQ
IAQYILDDYICSGQGGYANIYVTQPRRISAISVAERVARERCEQLGDTVG
YSVRFESVFPRPYGAILFCTVGVLLRKLEAGLRGVSHIIVDEIHERDVNS
DFLLVILRDMVDTYPDLHVILMSATIDTTKFSKYFGICPVLEVPGRAFPV
QQFFLEDIIQMTDFVPSAESRRKRKEVEDEEQLLSEDKDEAEINYNKVCE
DKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGAILVFLPGWNLI
FALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTKIILS
TNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRK
GRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEMALTIKLLRLGS
IHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPLGRLLARLPIEP
RLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQRRLANHQKALSG
TKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMSTMNVIWDAKQQ
LLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNICVHK
EKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQL
SMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELAAKIGALKPALE
DLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFGLQR

Ligand information

• Ligand ID: ALF
• InChI: InChI=1S/Al.4FH/h;4*1H/q+3;;;;/p-4
• InChIKey: UYOMQIYKOOHAMK-UHFFFAOYSA-J
• SMILES:
  ACDLabs 10.04
  CACTVS 3.341
  OpenEye OEToolkits 1.5.0: F[Al-](F)(F)F
• Formula: Al F4
• Name: TETRAFLUOROALUMINATE ION
• DrugBank: DB04444
• PDB chain: 5aor Chain B Residue 2161

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5aor Structure of the RNA Helicase Mle Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling.
• Resolution: 2.08 Å
• Binding residue (original residue number in PDB): T409 K413 E508 S723 R768 R771
• Binding residue (residue number reindexed from 1): T243 K247 E342 S557 R602 R605
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Gene Ontology

Molecular Function

• GO:0001069 regulatory region RNA binding
• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0003682 chromatin binding
• GO:0003690 double-stranded DNA binding
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0003725 double-stranded RNA binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0033679 3'-5' DNA/RNA helicase activity
• GO:0034458 3'-5' RNA helicase activity
• GO:0043138 3'-5' DNA helicase activity
• GO:0106222 lncRNA binding

Biological Process

• GO:0007549 sex-chromosome dosage compensation
• GO:0008340 determination of adult lifespan
• GO:0009047 dosage compensation by hyperactivation of X chromosome
• GO:0010468 regulation of gene expression
• GO:0031453 positive regulation of heterochromatin formation
• GO:0032508 DNA duplex unwinding
• GO:0042714 dosage compensation complex assembly
• GO:0045433 male courtship behavior, veined wing generated song production
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0048675 axon extension
• GO:0050684 regulation of mRNA processing
• GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
• GO:2000765 regulation of cytoplasmic translation

Cellular Component

• GO:0000228 nuclear chromosome
• GO:0000785 chromatin
• GO:0000805 X chromosome
• GO:0005634 nucleus
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0005829 cytosol
• GO:0016456 X chromosome located dosage compensation complex, transcription activating
• GO:0072487 MSL complex
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5aor, PDBe:5aor, PDBj:5aor
• PDBsum: 5aor
• PubMed: 26545078
• UniProt: P24785|MLE_DROME Dosage compensation regulator mle (Gene Name=mle)