Structure of PDB 3j94 Chain B Binding Site BS03

Receptor Information
>3j94 Chain B (length=484) Species: 10029 (Cricetulus griseus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGK
TLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRL
GANSGLHIIIFDEIHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEA
LLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVE
TKNFSGAELEGLVRAAQSTAMNRHIDFLASLENDIKPAFGTNQEDYASYI
MNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALA
AKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVD
DIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQ
EMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKV
WIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEG
Ligand information
Ligand IDATP
InChIInChI=1S/C10H16N5O13P3/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1
InChIKeyZKHQWZAMYRWXGA-KQYNXXCUSA-N
SMILES
SoftwareSMILES
OpenEye OEToolkits 1.5.0c1nc(c2c(n1)n(cn2)C3C(C(C(O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N
CACTVS 3.341Nc1ncnc2n(cnc12)[CH]3O[CH](CO[P](O)(=O)O[P](O)(=O)O[P](O)(O)=O)[CH](O)[CH]3O
ACDLabs 10.04O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O
OpenEye OEToolkits 1.5.0c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@@](=O)(O)O[P@](=O)(O)OP(=O)(O)O)O)O)N
CACTVS 3.341Nc1ncnc2n(cnc12)[C@@H]3O[C@H](CO[P@](O)(=O)O[P@@](O)(=O)O[P](O)(O)=O)[C@@H](O)[C@H]3O
FormulaC10 H16 N5 O13 P3
NameADENOSINE-5'-TRIPHOSPHATE
ChEMBLCHEMBL14249
DrugBankDB00171
ZINCZINC000004261765
PDB chain3j94 Chain C Residue 802 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB3j94 Mechanistic insights into the recycling machine of the SNARE complex.
Resolution4.2 Å
Binding residue
(original residue number in PDB)		R385 R388
Binding residue
(residue number reindexed from 1)		R153 R156
Annotation score5
Enzymatic activity
Catalytic site (original residue number in PDB) K549 T550 D603 D604 K631 K708
Catalytic site (residue number reindexed from 1) K296 T297 D350 D351 K378 K455
Enzyme Commision number 3.6.4.6: vesicle-fusing ATPase.
Gene Ontology
Molecular Function
GO:0000149 SNARE binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017075 syntaxin-1 binding
GO:0019901 protein kinase binding
GO:0019905 syntaxin binding
GO:0030165 PDZ domain binding
GO:0035255 ionotropic glutamate receptor binding
GO:0042802 identical protein binding
GO:0044877 protein-containing complex binding
GO:0046872 metal ion binding
GO:0140545 ATP-dependent protein disaggregase activity
Biological Process
GO:0001921 positive regulation of receptor recycling
GO:0006813 potassium ion transport
GO:0006886 intracellular protein transport
GO:0006891 intra-Golgi vesicle-mediated transport
GO:0015031 protein transport
GO:0035494 SNARE complex disassembly
GO:0043001 Golgi to plasma membrane protein transport
GO:0045732 positive regulation of protein catabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0005795 Golgi stack
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0030496 midbody

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:3j94, PDBe:3j94, PDBj:3j94
PDBsum3j94
PubMed25581794
UniProtP18708|NSF_CRIGR Vesicle-fusing ATPase (Gene Name=NSF)

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