Structure of PDB 2e8z Chain B Binding Site BS03

Receptor Information
>2e8z Chain B (length=712) Species: 1423 (Bacillus subtilis) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREE
YSLEAKYKYVCVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEF
YYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGV
YAVTVTGDLHGYEYLFCICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQM
KWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYLALTETDTQTA
NGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAP
EGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFE
KTVPGYFFRHDECGKPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNV
DGFRFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAA
LANAPRMPGIGFFNDMFRDAVKGNTFHLKATGFALGNGESAQAVMHGIAG
SSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQR
LAVAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFK
EDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDLDE
VDEWKDIIVIHHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAV
NGIGTVILYLAS
Ligand information
Ligand IDCA
InChIInChI=1S/Ca/q+2
InChIKeyBHPQYMZQTOCNFJ-UHFFFAOYSA-N
SMILES
SoftwareSMILES
CACTVS 3.341[Ca++]
ACDLabs 10.04
OpenEye OEToolkits 1.5.0		[Ca+2]
FormulaCa
NameCALCIUM ION
ChEMBL
DrugBankDB14577
ZINC
PDB chain2e8z Chain B Residue 742 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
Receptor-Ligand Complex Structure
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PDB2e8z Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168
Resolution2.2 Å
Binding residue
(original residue number in PDB)		D275 F276 E281 E301
Binding residue
(residue number reindexed from 1)		D275 F276 E281 E301
Annotation score4
Enzymatic activity
Catalytic site (original residue number in PDB) N188 D275 F276 E301 D406 E435 D525
Catalytic site (residue number reindexed from 1) N188 D275 F276 E301 D406 E435 D525
Enzyme Commision number 3.2.1.41: pullulanase.
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0051060 pullulanase activity
Biological Process
GO:0005975 carbohydrate metabolic process

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:2e8z, PDBe:2e8z, PDBj:2e8z
PDBsum2e8z
PubMed
UniProtC0SPA0|PULA_BACSU Pullulanase (Gene Name=amyX)

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