Structure of PDB 2e2i Chain B Binding Site BS03

Receptor Information
>2e2i Chain B (length=1143) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
DESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTL
ILEQYEISFGKIYVTKPMVNESDGVTHALYPQEARLRNLTYSSGLFVDVK
KRTYEAIDVPGRELKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDM
GGYFIINGSEKVLIAQERSAGNIVQVFKKAAPSPISHVAEIRSALEKGSR
FISTLQVKLYGREGSSARTIKATLPYIKQDIPIVIIFRALGIIPDGEILE
HICYDVNDWQMLEMLKPCVEDGFVIQDRETALDFIGRRGTALGIKKEKRI
QYAKDILQKEFLPHITQLEGFESRKAFFLGYMINRLLLCALDRKDQDDRD
HFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQRTVENMKLAINAKTIT
SGLKYALATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGR
DGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPI
ITFLSEWGMEPLEDYVPHQSPDATRVFVNGVWHGVHRNPARLMETLRTLR
RKGDINPEVSMIRDIREKELKIFTDAGRVYRPLFIVEDDESLGHKELKVR
KGHIAKLMATEYQDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLE
PAEDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNT
YQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLGTTRAMEYLKFRELP
AGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKKYGM
SITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVSGEDVIIGKTTPI
SSKRDASTPLRSTENGIVDQVLVTTNQDGLKFVKVRVRTTKIPQIGDKFA
SRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECLL
SKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKKL
MAQIFFGPTYYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFG
EMERDCMIAHGAASFLKERLMEASDAFRVHICGICGLMTVIAKLNHNQFE
CKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRLYTDRSRDF
Ligand information
Ligand IDZN
InChIInChI=1S/Zn/q+2
InChIKeyPTFCDOFLOPIGGS-UHFFFAOYSA-N
SMILES
SoftwareSMILES
CACTVS 3.341[Zn++]
ACDLabs 10.04
OpenEye OEToolkits 1.5.0		[Zn+2]
FormulaZn
NameZINC ION
ChEMBLCHEMBL1236970
DrugBankDB14532
ZINC
PDB chain2e2i Chain B Residue 1307 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
Receptor-Ligand Complex Structure
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PDB2e2i Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis
Resolution3.41 Å
Binding residue
(original residue number in PDB)		C1163 C1166 C1185
Binding residue
(residue number reindexed from 1)		C1082 C1085 C1104
Annotation score1
Enzymatic activity
Catalytic site (original residue number in PDB) D837
Catalytic site (residue number reindexed from 1) D769
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Gene Ontology
Molecular Function
GO:0001055 RNA polymerase II activity
GO:0003677 DNA binding
GO:0003729 mRNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0003968 RNA-dependent RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0001172 RNA-templated transcription
GO:0006351 DNA-templated transcription
GO:0006366 transcription by RNA polymerase II
GO:0006367 transcription initiation at RNA polymerase II promoter
GO:0006368 transcription elongation by RNA polymerase II
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005665 RNA polymerase II, core complex
GO:0005739 mitochondrion
GO:0010494 cytoplasmic stress granule

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:2e2i, PDBe:2e2i, PDBj:2e2i
PDBsum2e2i
PubMed17129781
UniProtP08518|RPB2_YEAST DNA-directed RNA polymerase II subunit RPB2 (Gene Name=RPB2)

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