Structure of PDB 8r6w Chain A Binding Site BS03

Receptor Information

>8r6w Chain A (length=1974) Species: 992210 (SFTS virus AH12)

MNLEVLCGRINVENGLSLGEPGLYDQIYDRPGLPDLDVTVDATGVTVDIG
AVPDSASQLGSSINAGLITIQLSEAYKINHDFTFSGLSKTTDRRLSEVFP
ITHDGSDGMTPAVIHTRLDGTIVVVEFSTTRSHNIGGLEAAYRTKIEKYR
DPISRRVDIMENPRVFFGVIVVSSGGVLSNMPLTQDEAEELMYRFCIANE
IYTKARSEELEAISRALSFFSLFEPNIERVEGTFPNSEIKMLEQFLSTPA
DVDFITKTLKAKEVEAYADLCDSHYLKPEKTIQERLEINRCEAIDKTQDL
LAGLHARSNKQTSLNRGTVKLPPWLPKPSSESIDIKTDSGFGSLMDHGAY
GELWAKCLLDVSLGNVEGVVSDPAKELDIAISDDPITYRRFKPALSSSAR
QEFSLQGVEGKKWKRMAANQKKEKESHETLSPFLDVEDIGDFLTFNNLLT
DSRYGDESIQRAVSILLEKASAMQDTELTHALNDSFKRNLSSNVVQWSLW
VSCLAQELASALKQHCRAGEFIIKKLKFWPIYVIIKPTKSSSHIFYSLGI
RKADVTRRLTGRVFSDTIDAGEWELTEFKSLKTCKLTNLVNLPCTMLNSI
AFWREKLGVAPWLVRKPCSELREQVGLTFLISLEDKSKTEEIITLTRYTQ
MEGFVSPPMLPKPQKMLGKLDGPLRTKLQVYLLRKHLDCMVRIASQPFSL
IPREGRVEWGGTFHAISGRSTNLENMVNSWYIGYYKNKEESTELNALGEM
YKKIVEMEEDKPSSPEFLGWGDTDSPKKHEFSRSFLRAACSSLEREIAQR
HGRQWKQNLEERVLREIGTKNILDLASMKATSNFSKDWELYSEVQTKEYH
RSKLLEKMATLIEKGVMWYIDAVGQAWKAVLDDGCMRICLFKKNQHGGLR
EIYVMDANARLVQFGVETMARCVCELSPHETVANPRLKNSIIENHGLKSA
RSLGPGSININSSNDAKKWNQGHYTTKLALVLCWFMPAKFHRFIWAAISM
FRRKKMMVDLRFLAHLSSKSESRSSDPFREAMTDAFHGNRDVSWMDKGRT
YIKTETGMMQGILHFTSSLLHSCVQSFYKSYFVSKLKEGYMGESISGVVD
VIEGSDDSAIMISIRPKSDMDEVRSRFFVANLLHSVKFLNPLFGIYSSEK
STVNTVYCVEYNSEFHFHRHLVRPTLRWIAASHQISETEALASRQEDYSN
LLTQCLEGGASFSLTYLIQCAQLLHHYMLLGLCLHPLFGTFMGMLISDPD
PALGFFLMDNPAFAGGAGFRFNLWRACKTTDLGRKYAYYFNEIQGKTKGD
EDYRALDATSGGTLSHSVMVYWGDRKKYQALLNRMGLPEDWVEQIDENPG
VLYRRAANKKELLLKLAEKVHSPGVTSSLSKGHVVPRVVAAGVYLLSRHC
FRFSTQKASLIKLLMMSSISAMKHGGSLNPNQERMLFPQAQEYDRVCTLL
EEVEHLTGKFVVRERNIVRSRIDLFQEPVDLRCKAEDLVSEVWFGLKRTK
LGPRLLKEEWDKLRASFAWLSTDPSETLRDGPFLSHVQFRNFIAHVDAKS
RSVRLLGAPVTTISQVVRMNFFPGFSLEAEESISILKHVLFMVLNGPYTE
EYKLEMIIEAFSTLVIPQPSEVIRKSRTMTLCLLSNYLSSRGGSILDQIE
RAQSGTLGGFSKPQKTFVRPGGGVGYKGKGVWTGVMEDTHVQILIDGDGT
SNWLEEIRLSSDARLYDVIESIRRLCDDLGINNRVASAYRGHCMVRLSGF
KIKPASRTDGCPVRIMNPDEVKMRVRGDILNLSVTIQEGRVMNILSYRPR
DTDISESAAAYLWSNRDLFSFGKKEPSCSWICLKTLDNWAWSHASVLLAN
DRKTQGIDNRAMGNIFRDCLEGSLRKQGLMRSKLIAVELSEGSLDIESIF
DGAPILWSAEVEEFGEGVVAVSYSSKYYHLTLMDQAAITMCAIMGKEGCR
GLLTEKRCMAAIREQVRPFLIFLQ

Ligand information

>8r6w Chain T (length=17)

cugggcggucuuugugu

.................

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8r6w Structural snapshots of phenuivirus cap-snatching and transcription
• Resolution: 3.35 Å
• Binding residue (original residue number in PDB): H535 R537 E759 K849 A850 H870 R871 F911 I922 R930 G1081 I1082 F1085 W1342 D1344 K1347 Y1348 K1401 G1402 H1403
• Binding residue (residue number reindexed from 1): H515 R517 E739 K829 A830 H850 R851 F891 I902 R910 G1061 I1062 F1065 W1322 D1324 K1327 Y1328 K1381 G1382 H1383

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016740 transferase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:8r6w, PDBe:8r6w, PDBj:8r6w
• PDBsum: 8r6w
• PubMed: 38709882
• UniProt: I0DF35|L_SFTS RNA-directed RNA polymerase L