Structure of PDB 8pj9 Chain A Binding Site BS03

Receptor Information

>8pj9 Chain A (length=1051) Species: 1268061 (Streptococcus thermophilus DGCC 7710)

KPYSIGLDIGTNSVGWAVITDNYKVPSKKMKVLGNTSKKYIKKNLLGVLL
FDSGITAEGRRLKRTARRRYTRRRNRILYLQEIFSTEMATLDDAFFQRLD
DSFLVPDDKRDSKYPIFGNLVEEKVYHDEFPTIYHLRKYLADSTKKADLR
LVYLALAHMIKYRGHFLIEGEFNVTDNETEAPLSSAMIKRYNEHKEDLAL
LKEYIRNISLKTYNEVFKDDTKNGYAGYIDGKTNQEDFYVYLKNLLAEFE
GADYFLEKIDREDFLRKQRTFDNGSIPYQIHLQEMRAILDKQAKFYPFLA
KNKERIEKILTFRIPYYVGPLARGNSDFAWSIRKRNEKITPWNFEDVIDK
ESSAEAFINRMTSFDLYLPEEKVLPKHSLLYETFNVYNELTKVRFIAESM
RDYQFLDSKQKKDIVRLYFKDKRKVTDKDIIEYLHAIYGYDGIELKGIEK
QFNSSLSTYHDLLNIINDKEFLDDSSNEAIIEEIIHTLTIFEDREMIKQR
LSKFENIFDKSVLKKLSRRHYTGWGKLSAKLINGIRDEKSGNTILDYLID
DGISNRNFMQLIHDDALSFKKKIQKAQIIGDEDKGNIKEVVKSLPGSPAI
KKGILQSIKIVDELVKVMGGRKPESIVVEMATKHVARLLDEKFNNKKDEN
NRAVRTVKIITLKSTLVSQFRKDFELYKVREINDFHHAHDAYLNAVIASA
LLKKYPKLEPEFVYGSNIMNIFKKSRPLIEVNEETGESVWNKESDLATVR
RVLSYPQVNVVKKVEEQNHGLDRGKPKGLFNANLSSKPKPNSNENLVGAK
EYLDPKKYGGYAGISNSFAVLVKGTIEKGAKKKITNVLEFQGISILDRIN
YRKDKLNFLLEKGYKDIELIIELPKYSLFELSDGSRRMLASILSTNNKRG
EIHKGNQIFLSQKFVKLLYHAKRISNTINENHRKYVENHKKEFEELFYYI
LEFNENYVGAKKNGKLLNSAFQSWQNHSIDELCSSFIGPTGSERKGLFEL
TSRGSAADFEFLGVKIPRYRDYTPSSLLKDATLIHQSVTGLYETRIDLAK
L

Ligand information

>8pj9 Chain D (length=13)

tgactcaccatgt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8pj9 Structural basis for spacer acquisition in a type II-A CRISPR-Cas system
• Resolution: 3.24 Å
• Binding residue (original residue number in PDB): R1107 N1115 A1116 N1117 D1355
• Binding residue (residue number reindexed from 1): R773 N781 A782 N783 D1021

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:8pj9, PDBe:8pj9, PDBj:8pj9
• PDBsum: 8pj9
• UniProt: G3ECR1|CAS9_STRTR CRISPR-associated endonuclease Cas9 (Gene Name=cas9)