Structure of PDB 8jtj Chain A Binding Site BS03

Receptor Information

>8jtj Chain A (length=1042) Species: 1422 (Geobacillus stearothermophilus)

MRYKIGLDIGITSVGWAVMNLDIPRIEDLGVRIFDRAENPQTGESLALPR
RLARSARRRLRRRKHRLERIRRLVIREGILTKEELDKLFEEKHEIDVWQL
RVEALDRKLNNDELARVLLHLAKRRGFKSNRKSKENSTMLKHIEENRAIL
SSYRTVGEMIVKDPKFALHKRNKGENYTNTIARDDLEREIRLIFSKQREF
GNMSCTEEFENEYIAIWASQRPVASKDDIEKKVGFCAFEPKEKRAPKATY
TFQSFIAWEHINKLRLISPSGARGLTDEERRLLYEQAFQKNKITYHDIRT
LLHDTYFKGIVYDRQNENIRFLELDAYHQIRKAVDKVYGKEKSSSFLPID
FDTFGYALTLFKDDADIHSYLRNEYEQNGKRMPNLANKVYDNELIEELLN
LSFTKFGHLSLKALRSILPYMEQGEVYSSACERAGYTFTGPKKKQKTMLL
PNIPPIANPVVMRALTQARKVVNAIIKKYGSPVSIHIELARDLSQTFDER
RKTKKEQDENRKKNETAIRQLMEYGLTLNPTGHDIVKFKLWSEQNGQPIE
IERLLEPGYVEVDAVIPYSRSLDDSYTNKVLVLTREREKGNRIPAEYLGF
ETFVLTNSKKKRDRLLRLHYDENEETEFKNRNLNDTRYISRFFANFIREH
LKFAESDDKQKVYTVNGRVTAHLRSRWEFNKNREESDLHHAVDAVIVACT
TPSDIAKVTAFYQRREQNKELAKKTEPHFPQPWPHFADELRARLSKHPKE
SIKALNLGNYDDQKLESLQPVFVSRMPKRSVTGAAHQETLRRYVGIDERS
GKIQTVVKTKLSEIKLDASGHFPMYGKESDPRTYEAIRQRLLDPKKAFQE
PLYKPKKNGEPGPVIRTVKIIDTKNQVIPLNDGKTVAYNSNIVRVDVFEK
DGKYYCVPVYTMDIMKGILPNKAIEPNKPYSEWKEMTEDYTFRFSLYPND
LIRIELPREKTVKTAAGEEINVKDVFVYYKTIDSANGGLELISHDHRFSL
RGVGSRTLKRFEKYQVDVLGNIYKVRGEKRVGLASSAHSKPG

Ligand information

>8jtj Chain D (length=9)

gggcgcgaa

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8jtj Cryo-EM structure of GeoCas9-sgRNA-DNA ternary complex
• Resolution: 3.08 Å
• Binding residue (original residue number in PDB): P40 Q41 K908 N909 N925 I926 N961 T1015 D1017 S1018
• Binding residue (residue number reindexed from 1): P40 Q41 K874 N875 N891 I892 N927 T981 D983 S984

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:8jtj, PDBe:8jtj, PDBj:8jtj
• PDBsum: 8jtj
• UniProt: A0A150MP45