Structure of PDB 8j1j Chain A Binding Site BS03

Receptor Information
>8j1j Chain A (length=424) Species: 1765684 (Sulfoacidibacillus thermotolerans) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
GSMIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGY
SSDYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRA
TDRFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSL
LSNPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVHASQIIHDDRK
NKWYLNISYDFEPQTRVLDLNKIMGIDLGVAVAAYMAFQHTPARYKLEGG
EIENFRRQVESRRISMLRQGKYAGGARGGHGRDKRIKPIEQLRDKIANFR
DTTNHRYSRYIVDMAIKMGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQK
IIYKAEEAGIKVIKIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEA
NADYNAARNIAIPNIDKIIAESIK
Ligand information
>8j1j Chain C (length=112) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ucgucgguucagcgacgauaagccgagaagugccaauaaaacuguuaagu
gguuugguaacgcucgguaagguccgaaaggagaaccacugaacggaaau
uaggugcgcuug
<<<<<<.(((((>>>>>>...<<<<<<..<<<<<<<....<<<....>>>
...>>>>>.>>.>>>>>>..<<<<<....>>...>>>.))))).......
............
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8j1j Minimal and most efficient genome editing Cas enzyme
Resolution2.91 Å
Binding residue
(original residue number in PDB)		I2 K3 V4 Y5 E8 K11 W17 R101 M114 S115 S118 Y119 K120 P124 I168 R170 A172 Q191 K198 K200 Y202 R260 S263 M264 L265 Q267 K269 G272 A274 G277 H278 G279 R280 K282 R283 I284 K285 P286 Q289 K293 N296 T300 H303 R304 R307 Y351 K352
Binding residue
(residue number reindexed from 1)		I4 K5 V6 Y7 E10 K13 W19 R103 M116 S117 S120 Y121 K122 P126 I170 R172 A174 Q193 K200 K202 Y204 R262 S265 M266 L267 Q269 K271 G274 A276 G279 H280 G281 R282 K284 R285 I286 K287 P288 Q291 K295 N298 T302 H305 R306 R309 Y353 K354
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding

View graph for
Molecular Function
External links
PDB RCSB:8j1j, PDBe:8j1j, PDBj:8j1j
PDBsum8j1j
PubMed
UniProtA0A2U3D0N8|CS12F_SULT2 CRISPR-associated endodeoxyribonuclease Cas12f1 (Gene Name=cas12f1)

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