Structure of PDB 8efy Chain A Binding Site BS03

Receptor Information

>8efy Chain A (length=313) Species: 300852 (Thermus thermophilus HB8)

ALRPKTLDEYIGQERLKQKLRVYLEAAKARKEPLEHLLLFGPPGLGKTTL
AHVIAHELGVNLRVTSGPAIEKPGDLAAILANSLEEGDILFIDEIHRLSR
QAEEHLYPAMEDFVMDIVIGQGPAARTIRLELPRFTLIGATTRPGLITAP
LLSRFGIVEHLEYYTPEELAQGVMRDARLLGVRITEEAALEIGRRSRGTM
RVAKRLFRRVRDFAQVAGEEVITRERALEALAALGLDELGLEKRDREILE
VLILRFGGGPVGLATLATALSEDPGTLEEVHEPYLIRQGLLKRTPRGRVA
TELAYRHLGYPPP

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 8efy Chain A Residue 401

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8efy Structure of double homo-hexameric AAA+ ATPase RuvB motor binding with DNA substrate
• Resolution: 3.16 Å
• Binding residue (original residue number in PDB): D97 E98
• Binding residue (residue number reindexed from 1): D93 E94
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Gene Ontology

Molecular Function

• GO:0000400 four-way junction DNA binding
• GO:0003677 DNA binding
• GO:0005524 ATP binding
• GO:0009378 four-way junction helicase activity
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0032508 DNA duplex unwinding

Cellular Component

• GO:0005737 cytoplasm
• GO:0048476 Holliday junction resolvase complex

External links

• PDB: RCSB:8efy, PDBe:8efy, PDBj:8efy
• PDBsum: 8efy
• UniProt: Q5SL87|RUVB_THET8 Holliday junction branch migration complex subunit RuvB (Gene Name=ruvB)