Structure of PDB 8d4a Chain A Binding Site BS03

Receptor Information

>8d4a Chain A (length=1207) Species: 1539066 (Sulfuricurvum sp. PC08-66)

MLHAFTNQYQLSKTLRFGATLKEDEKKCKSHEELKGFVDISYENMKSSAT
ENELVKKCERCYSEIVKFHNAWEKIYYRTDQIAVYKDFYRQLSRKARFDA
GKQNSQLITLASLCGMYQGAKLSRYITNYWKDNITRQKSFLKDFSQQLHQ
YTRALEKSDKAHTKPNLINFNKTFMVLANLVNEIVIPLSNGAISFPNISK
LEDGEESHLIEFALNDYSQLSELIGELKDAIATNGGYTPFAKVTLNHYTA
EQKPHVFKNDIDAKIRELKLIGLVETLKGKSSEQIEEYFSNLDKFSTYND
RNQSVIVRTQCFKYKPIPFLVKHQLAKYISEPNGWDEDAVAKVLDAVGAI
RSPAHDYANNQEGFDLNHYPIKVAFDYAWEQLANSLYTTVTFPQEMCEKY
LNSIYGCEVSKEPVFKFYADLLYIRKNLAVLEHKNNLPSNQEEFICKINN
TFENIVLPYKISQFETYKKDILAWINDGHDHKKYTDAKQQLGFIRGGLKG
RINPYTKLTNEFKQISSTYGKTFAELRDKFKEKNEITKITHFGIIIEDKN
RDRYLLASELKHEQINHVSTILNKLDKSSEFITYQVKSLTSKTLIKLIKN
HTTKKGAISPYADFHTSKTGFNKNEIEKNWDNYKREQVLVEYVKDCLTDS
TMAKNQNWAEFGWNFEKCNSYEDIEHEIDQKSYLLQSDTISKQSIASLVE
GGCLLLPIINQDITSKERKDKNQFSKDWNHIFEGSKEFRLHPEFAVSYRT
PIEGYPVQKRYGRLQFVCAFNAHIVPQNGEFINLKKQIENFNDEDVQKRN
VTEFNKKVNHALSDKEYVVIGIDRGLKQLATLCVLDKRGKILGDFEIYKK
EFVRAEKRSESHWEHTQAETRHILDLSNLRVETTIEGKKVLVDQSLTLVK
KNRDTPDEEATEENKQKIKLKQLSYIRKLQHKMQTNEQDVLDLINNEPSD
EEFKKRIEGLISSFGEGQKYADLPINTMREMISDLQGVIARGNNQTEKNK
IIELDAADNLKQGIVANMIGIVNYIFAKYSYKAYISLEDLSRAYGGAKSG
YDGRYLPSTSQDEDVDFKEQQNQMLAGLGTYQFFEMQLLKKLQKIQSDNT
VLRFVPAFRSADNYRNILRLEETKYKSKPFGVVHFIDPKFTSKKCPVCSK
TNVYRDKDDILVCKECGFRSDSQLKERENNIHYIHNGDDNGAYHIALKSV
ENLIQMK

Ligand information

>8d4a Chain D (length=11)

ttttttttttt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8d4a RNA targeting unleashes indiscriminate nuclease activity of CRISPR-Cas12a2.
• Resolution: 2.74 Å
• Binding residue (original residue number in PDB): L851 K852 G992 Q993 K994 L1065 R1067 A1068 Y1069 F1092 Q1096 P1163 F1165 S1167
• Binding residue (residue number reindexed from 1): L826 K827 G967 Q968 K969 L1040 R1042 A1043 Y1044 F1067 Q1071 P1138 F1140 S1142

Gene Ontology

Molecular Function

• GO:0003677 DNA binding

External links

• PDB: RCSB:8d4a, PDBe:8d4a, PDBj:8d4a
• PDBsum: 8d4a
• PubMed: 36599980
• UniProt: A0A0C2W1L1