Structure of PDB 7sxe Chain A Binding Site BS03

Receptor Information

>7sxe Chain A (length=638) Species: 9606 (Homo sapiens)

LDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVE
TLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLALGVGDGVLLKAVAQ
ATGRQLESVRAEAAEKGDVGLVAENSRMLPPPPLTASGVFSKFRDIARLT
GSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQ
AVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDR
IIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTC
EYKYDGQRAQIHALEGGEVKIFSRNQADNTGKYPDIISRIPKIKLPSVTS
FILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYL
NGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSV
KDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAY
LGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKAL
VLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSD
KGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQI

Ligand information

>7sxe Chain D (length=18)

gtccgactacgcatcagc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7sxe Structures of LIG1 that engage with mutagenic mismatches inserted by pol beta in base excision repair.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): R305 T415 G416 S417 A418 S419 T420 R451 G453 A455 Q457 S458 Q636 T639 T640 R641 R643 R768 K770 R771 G776 C794 K795 G797 S850 S852 P853 Y855 S869 L870
• Binding residue (residue number reindexed from 1): R45 T150 G151 S152 A153 S154 T155 R186 G188 A190 Q192 S193 Q371 T374 T375 R376 R378 R503 K505 R506 G511 C529 K530 G532 S585 S587 P588 Y590 S604 L605

Enzymatic activity

• Enzyme Commision number: 6.5.1.1: DNA ligase (ATP).

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003909 DNA ligase activity
• GO:0003910 DNA ligase (ATP) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0071897 DNA biosynthetic process

External links

• PDB: RCSB:7sxe, PDBe:7sxe, PDBj:7sxe
• PDBsum: 7sxe
• PubMed: 35790757
• UniProt: P18858|DNLI1_HUMAN DNA ligase 1 (Gene Name=LIG1)