Structure of PDB 7sx5 Chain A Binding Site BS03

Receptor Information

>7sx5 Chain A (length=637) Species: 9606 (Homo sapiens)

LDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVE
TLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLALGVGDGVLLKAVAQ
ATGRQLESVRAEAAEKGDVGLVAENSRRLMLPPPPLTASGVFSKFRDIAR
LTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAAL
SQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDL
DRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAF
TCEYKYDGQRAQIHALEGGEVKIFSRNQADNTGKYPDIISRIPKIKLPSV
TSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLI
YLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQ
SVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLGVGDTLDLVVIGA
YLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKA
LVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDS
DKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQS

Ligand information

>7sx5 Chain D (length=18)

gtccgaccacgcatcagc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7sx5 Structures of LIG1 that engage with mutagenic mismatches inserted by pol beta in base excision repair.
• Resolution: 2.8 Å
• Binding residue (original residue number in PDB): R305 G416 A418 T420 R449 R451 L452 G453 A455 Q457 S458 Q636 T639 T640 R641 K642 R643 K644 R768 K795 G797 T798 S850 S852 P853 Y855 S869 L870 F872
• Binding residue (residue number reindexed from 1): R45 G153 A155 T157 R186 R188 L189 G190 A192 Q194 S195 Q373 T376 T377 R378 K379 R380 K381 R504 K531 G533 T534 S586 S588 P589 Y591 S605 L606 F608

Enzymatic activity

• Enzyme Commision number: 6.5.1.1: DNA ligase (ATP).

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003909 DNA ligase activity
• GO:0003910 DNA ligase (ATP) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0071897 DNA biosynthetic process

External links

• PDB: RCSB:7sx5, PDBe:7sx5, PDBj:7sx5
• PDBsum: 7sx5
• PubMed: 35790757
• UniProt: P18858|DNLI1_HUMAN DNA ligase 1 (Gene Name=LIG1)