Structure of PDB 6gtg Chain A Binding Site BS03

Receptor Information
>6gtg Chain A (length=1298) Species: 401614 (Francisella tularensis subsp. novicida U112) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
IYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQ
IIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAK
DTIKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIEL
FKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYR
IVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYKTSE
VNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGINE
YINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTM
QSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDL
SQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLS
LETIKLALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQI
SIKYQNQGKKDLLQASAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKA
NILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNFEN
STLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGE
GYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGS
PQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDE
FYREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPN
LHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANK
NKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEINL
LLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTN
YHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAI
VVFQDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVL
RAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESV
SKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLI
NFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKK
FFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQ
DADANGAYHIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRNN
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB6gtg Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Resolution3.27 Å
Binding residue
(original residue number in PDB)		N124 Q125 G174 T177 N666 K667 K671 T698 K699 D751 K895
Binding residue
(residue number reindexed from 1)		N122 Q123 G172 T175 N664 K665 K669 T696 K697 D749 K893
Enzymatic activity
Enzyme Commision number 3.1.21.1: deoxyribonuclease I.
4.6.1.22: Bacillus subtilis ribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004530 deoxyribonuclease I activity
GO:0016829 lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6gtg, PDBe:6gtg, PDBj:6gtg
PDBsum6gtg
PubMed30503205
UniProtA0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

[Back to BioLiP]