Structure of PDB 6bk8 Chain A Binding Site BS03

Receptor Information
>6bk8 Chain A (length=1960) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
NLYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKL
LENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMR
REKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEY
VKDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDK
NYYYLFDKKSFFNGKALNNAIPGGPKFEPLYPRENEFNSIDRVIFRVPIR
SEYKVAFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLNP
IPHGDFTLPEDFAPLLAEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRA
QDVALAKKWFLQHPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTK
LLKSLKNTKYFQQTTIDWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYN
FNLKPTKTLTTKERKKSRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDA
FQLADGIHYILNHIGQLTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLG
KGPGCGFWQPAWRVWLNFLRGTIPLLERYIGNLITRQFEGRSNEIVKTTT
KQRLDAYYDLELRNSVMDDILEMMPESIRQKKARTILQHLSEAWRCWKAN
IPWDVPGMPAPIKKIIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVK
KNLGRLTRLWIKNEQERQRQIQKNGPEITPEEATTIFSVMVEWLESRSFS
PIPFPPLTYKNDTKILVLALEDLKDVYASKVRLNASEREELALIEEAYDN
PHDTLNRIKKYLLTQRVFKPVDITMMENYQNISPVYSVDPLEKITDAYLD
QYLWYEADQRKLFPNWIKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQS
AVLLETTLGEMAEKIDFTLLNRLLRLIVDPNIADYITAKNNVVINFKDMS
HVNKYGLIRGLKFASFIFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQ
FKSKEVEKAHPIRLYTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFE
NSIGYNNRKCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENA
FVSVYSKNNPNLLFSMCGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQR
TAKAYLKVSEEEIKKFDSRIRGILMASGSTTFTKVAAKWNTSLISLFTYF
REAIVATEPLLDILVKGETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGG
LGMISASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITT
WENEFLDSQRVWAEYATKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQ
RDRHTLAYDRGHRIRREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDV
IQALGGIETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRT
GLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLI
QIFRAHLWQKIHESIVFDICQILDGELDVLQIESVTKETVHPRKSYKMNS
SAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDVQLRYGDYDSH
DISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFNGLKP
LIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLNSSNYAELFNN
DIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHT
SVWAGQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVTRKAMLDPLEV
HMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWL
DRISSYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKSYHLWPSFTDE
QWITIESQMRDLILTEYGRKYNVNISALTQTEIKDIILGQNIKAPSVKRQ
KMAELEAARS
Ligand information
>6bk8 Chain 6 (length=102) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guucgcgaaguaacccuucguggacauuuggucaauuugaaacaauacag
agaugaucagcaguuccccugcauaaggaugaaccguuuuacaaagagau
uu
<<<<<<<<<<.....>>>>>>>>>>.........................
............<<<..<<<.....>>>...>>>................
..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6bk8 Structure of the yeast spliceosomal postcatalytic P complex.
Resolution3.3 Å
Binding residue
(original residue number in PDB)		S151 K152 M153 K608 E609 K611 R614 G616 S618 Y725 N728 R732 R737 K743 T744 Q748 R749 Y753 T1591 H1592 H1863 E1867 N1869 A1900 K1903
Binding residue
(residue number reindexed from 1)		S26 K27 M28 K462 E463 K465 R468 G470 S472 Y579 N582 R586 R591 K597 T598 Q602 R603 Y607 T1445 H1446 H1717 E1721 N1723 A1754 K1757
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000386 second spliceosomal transesterification activity
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0097157 pre-mRNA intronic binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000350 generation of catalytic spliceosome for second transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000389 mRNA 3'-splice site recognition
GO:0000395 mRNA 5'-splice site recognition
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6bk8, PDBe:6bk8, PDBj:6bk8
PDBsum6bk8
PubMed29146870
UniProtP33334|PRP8_YEAST Pre-mRNA-splicing factor 8 (Gene Name=PRP8)

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