Structure of PDB 5nfv Chain A Binding Site BS03

Receptor Information
>5nfv Chain A (length=1258) Species: 401614 (Francisella tularensis subsp. novicida U112) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
NASIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKK
AKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFK
SAKDTIKKQISEYIKDSEKFKNLFNQNLIDAKKSDLILWLKQSKDNGIEL
FKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYR
IVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYKTSE
VNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGINE
YINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTM
QSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDL
SQQVFDDYSVIGTAVLEYITQKTEKAKYLSLETIKLALEEFNKHRDIDKQ
CRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQASAEDD
VKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFEL
ANIVPLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILF
IKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKV
FFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIEDCRKFID
FYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLTFENISES
YIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDERNLQDVVY
KLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDKR
FTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERH
LAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWK
KINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFQDLKVEKQVYQKLEKML
IEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGF
TSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEFSFDYK
NFGDKAAKGKWTIASFGSRLINFRNDTREVYPTKELEKLLKDYSIEYGHG
ECIKAAICGESDKKFFAKLTSVLNTILQMANSKTDYLISPVADVNGNFFD
SRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYF
EFVQNRNN
Ligand information
Receptor-Ligand Complex Structure
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PDB5nfv Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.
Resolution2.501 Å
Binding residue
(original residue number in PDB)		N124 Q125 G174 T176 T177 K471 K639 N666 K667 K671 T698 K699 N700 G701 S702 D751 Y754 R755
Binding residue
(residue number reindexed from 1)		N125 Q126 G172 T174 T175 K449 K617 N644 K645 K649 T676 K677 N678 G679 S680 D729 Y732 R733
Enzymatic activity
Enzyme Commision number 3.1.21.1: deoxyribonuclease I.
4.6.1.22: Bacillus subtilis ribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004530 deoxyribonuclease I activity
GO:0016829 lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:5nfv, PDBe:5nfv, PDBj:5nfv
PDBsum5nfv
PubMed28431230
UniProtA0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

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