Structure of PDB 5mq0 Chain A Binding Site BS03

Receptor Information
>5mq0 Chain A (length=1914) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
LYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKLL
ENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMRR
EKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYV
KDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDKN
YYYLFDKKSFFNGKALNNAIPGGPKFEPLYPFNSIDRVIFRVPIRSEYKV
AFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLEDFAPLL
AEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRAQDVALAKKWFLQHPDE
EYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLLKSLKNTKYFQQTTI
DWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKK
SRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDAFQLADGIHYILNHIGQ
LTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLGKGPGCGFWQPAWRVWL
NFLRGTIPLLERYIGNLITRQFEGRSNEIVKTTTKQRLDAYYDLELRNSV
MDDILEMMPESIRQKKARTILQHLSEAWRCWKANIPWDVPGMPAPIKKII
ERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLWIKNEQE
RQRQIQKNGPEITPEEATTIFSVMVEWLESRSFSPIPFPPLTYKNDTKIL
VLALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLTQR
VFKPVDITMMENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFPNW
IKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQSAVLLETTLGEMAEKID
FTLLNRLLRLIVDPNIADYITAKNNVVINFKDMSHVNKYGLIRGLKFASF
IFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQFKSKEVEKAHPIRLYT
RYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIGYNNRKCWPKDSR
MRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLFSM
CGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQRTAKAYLKVSEEEIKKF
DSRIRGILMASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDILVK
GETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMISASHILIPASDL
SWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITTWENEFLDSQRVWAEYA
TKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQRDRHTLAYDRGHRIRR
EFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDVIQALGGIETILEHTLF
KGTGFNSWEGLFWQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHG
KIPTLKISLIQIFRAHLWQKIHESIVFDICQILDGELDVLQIESVTKETV
HPRKSYKMNSSAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDV
QLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDA
YGNWFNGLKPLIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLN
SSNYAELFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKT
GHLFLKIIHTSVWAGQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVT
RKAMLDPLEVHMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQM
VLFNIYDDWLDRISSYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKS
YHLWPSFTDEQWITIESQMRDLILTEYGRKYNVNISALTQTEIKDIILGQ
NIKAPSVKRQKMAE
Ligand information
>5mq0 Chain 2 (length=155) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
acgaaucucuuugccuuuuggcuuagaucaaguguaguaucuguucuuug
uaacaacugaaaugaccuaggcucauuguuacggacgggaagaggagacg
ucgcgacccucgcagucguucuugacuggucgcuugauguuucuucuucc
cguuc
.................................................<
<<<<<<......<<<<<<>>>.>>>>>>>>>><<<<<<<<<<<.<<<<<<
<<<<<<.<<.....<<<<....>>>>.>>>>>>..>>>>>>>>.>>>>>>
>>>>>
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5mq0 Structure of a spliceosome remodelled for exon ligation.
Resolution4.17 Å
Binding residue
(original residue number in PDB)		D751 D755 R759 R780 Q784 S787 W790 K794 K819 K846 K847 R851 R854 N930 A931 K1093 D1094 K1864
Binding residue
(residue number reindexed from 1)		D589 D593 R597 R618 Q622 S625 W628 K632 K657 K684 K685 R689 R692 N768 A769 K931 D932 K1678
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000386 second spliceosomal transesterification activity
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0097157 pre-mRNA intronic binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000350 generation of catalytic spliceosome for second transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000389 mRNA 3'-splice site recognition
GO:0000395 mRNA 5'-splice site recognition
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5mq0, PDBe:5mq0, PDBj:5mq0
PDBsum5mq0
PubMed28076345
UniProtP33334|PRP8_YEAST Pre-mRNA-splicing factor 8 (Gene Name=PRP8)

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