Structure of PDB 5b43 Chain A Binding Site BS03

Receptor Information
>5b43 Chain A (length=1272) Species: 1111120 (Acidaminococcus sp. BV3L6) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKEL
KPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQA
TYRNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLTTTE
HENALLRSFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKE
NCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQT
QIDLYNQLLGGISREAGTEKIKGLNEVLNLAIQKNDETAHIIASLPHRFI
PLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALF
NELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGKITK
SAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQP
LPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGI
KLEMEPSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNG
AILFVKNGLYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAK
MIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPK
KFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQ
YKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMEKMLNKKL
KDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKD
RRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGE
RNLIYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVV
GTIKDLKQGYLSQVIHEIVDLMIHYQAVVVLENLAVYQQFEKMLIDKLNC
LVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDP
LTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSF
QRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPYRDLYPANELIAL
LEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMRNSNAATGED
YINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
DLKLQNGISNQDWLAYIQELRN
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5b43 Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
Resolution2.8 Å
Binding residue
(original residue number in PDB)		K134 A135 K164 F165 T166 T167 R574 Y575 K603 K607 Q611
Binding residue
(residue number reindexed from 1)		K134 A135 K161 F162 T163 T164 R571 Y572 K600 K604 Q608
Enzymatic activity
Enzyme Commision number 3.1.21.1: deoxyribonuclease I.
4.6.1.22: Bacillus subtilis ribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004530 deoxyribonuclease I activity
GO:0016829 lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:5b43, PDBe:5b43, PDBj:5b43
PDBsum5b43
PubMed27114038
UniProtU2UMQ6|CS12A_ACISB CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

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