Structure of PDB 1s0v Chain A Binding Site BS03

Receptor Information

>1s0v Chain A (length=857) Species: 10760 (Escherichia phage T7)

NTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARF
RKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPT
AFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARF
GRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAW
SSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYA
EAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHS
KKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVED
IPAIEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFP
YNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANC
AGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE
YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETV
QDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALA
GQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMF
TQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGE
ILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLGQFRDSEIDAHKQES
GIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFK
AVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDIL
ESDFAFA

Ligand information

>1s0v Chain G (length=9)

gtcgattcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1s0v Structural basis for substrate selection by t7 RNA polymerase.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): F644 R647
• Binding residue (residue number reindexed from 1): F627 R630

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription

External links

• PDB: RCSB:1s0v, PDBe:1s0v, PDBj:1s0v
• PDBsum: 1s0v
• PubMed: 15016373
• UniProt: P00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)