Structure of PDB 1lan Chain A Binding Site BS03

Receptor Information
>1lan Chain A (length=484) Species: 9913 (Bos taurus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
TKGLVLGIYSKEKEEDEPQFTSAGENFNKLVSGKLREILNISGPPLKAGK
TRTFYGLHEDFPSVVVVGLGKKTAGIDEQENWHEGKENIRAAVAAGCRQI
QDLEIPSVEVDPCGDAQAAAEGAVLGLYEYDDLKQKRKVVVSAKLHGSED
QEAWQRGVLFASGQNLARRLMETPANEMTPTKFAEIVEENLKSASIKTDV
FIRPKSWIEEQEMGSFLSVAKGSEEPPVFLEIHYKGSPNASEPPLVFVGK
GITFDSGGISIKAAANMDLMRADMGGAATICSAIVSAAKLDLPINIVGLA
PLCENMPSGKANKPGDVVRARNGKTIQVDNTDAEGRLILADALCYAHTFN
PKVIINAATLTGAMDIALGSGATGVFTNSSWLWNKLFEASIETGDRVWRM
PLFEHYTRQVIDCQLADVNNIGKYRSAGACTAAAFLKEFVTHPKWAHLDI
AGVMTNKDEVPYLRKGMAGRPTRTLIEFLFRFSQ
Ligand information
Ligand IDLEU
InChIInChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1
InChIKeyROHFNLRQFUQHCH-YFKPBYRVSA-N
SMILES
SoftwareSMILES
OpenEye OEToolkits 1.5.0CC(C)C[C@@H](C(=O)O)N
CACTVS 3.341CC(C)C[C@H](N)C(O)=O
OpenEye OEToolkits 1.5.0CC(C)CC(C(=O)O)N
ACDLabs 10.04O=C(O)C(N)CC(C)C
CACTVS 3.341CC(C)C[CH](N)C(O)=O
FormulaC6 H13 N O2
NameLEUCINE
ChEMBLCHEMBL291962
DrugBankDB00149
ZINCZINC000003645145
PDB chain1lan Chain A Residue 500 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
Receptor-Ligand Complex Structure
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PDB1lan Two-metal ion mechanism of bovine lens leucine aminopeptidase: active site solvent structure and binding mode of L-leucinal, a gem-diolate transition state analogue, by X-ray crystallography.
Resolution1.9 Å
Binding residue
(original residue number in PDB)		D255 D273 D332 E334 T359 L360
Binding residue
(residue number reindexed from 1)		D255 D273 D332 E334 T359 L360
Annotation score2
Binding affinityMOAD: Ki=60nM
PDBbind-CN: -logKd/Ki=7.22,Ki=60nM
Enzymatic activity
Catalytic site (original residue number in PDB) K262 R336
Catalytic site (residue number reindexed from 1) K262 R336
Enzyme Commision number 3.4.11.1: leucyl aminopeptidase.
3.4.11.5: prolyl aminopeptidase.
3.4.13.23: cysteinylglycine-S-conjugate dipeptidase.
Gene Ontology
Molecular Function
GO:0004177 aminopeptidase activity
GO:0004180 carboxypeptidase activity
GO:0008233 peptidase activity
GO:0016805 dipeptidase activity
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
GO:0070006 metalloaminopeptidase activity
GO:0097718 disordered domain specific binding
Biological Process
GO:0006508 proteolysis
GO:0019538 protein metabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1lan, PDBe:1lan, PDBj:1lan
PDBsum1lan
PubMed7578088
UniProtP00727|AMPL_BOVIN Cytosol aminopeptidase (Gene Name=LAP3)

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