Structure of PDB 1k4t Chain A Binding Site BS03

Receptor Information

>1k4t Chain A (length=565) Species: 9606 (Homo sapiens)

QKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLS
PKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKC
DFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERI
ANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHK
WKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRL
KKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETAD
TVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKN
LQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQ
QLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTK
IDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMK
LEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQRE
KFAWAIDMADEDYEF

Ligand information

>1k4t Chain D (length=22)

aaaaatttttccaagtcttttt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1k4t The mechanism of topoisomerase I poisoning by a camptothecin analog
• Resolution: 2.1 Å
• Binding residue (original residue number in PDB): F361 R362 G363 R364 H367 K374 R488 A489 N491 K493 T501 K532 D533 N574 T585 A586 K587 N646
• Binding residue (residue number reindexed from 1): F161 R162 G163 R164 H167 K174 R288 A289 N291 K293 T301 K332 D333 N374 T385 A386 K387 N446

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1k4t, PDBe:1k4t, PDBj:1k4t
• PDBsum: 1k4t
• PubMed: 12426403
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)