Structure of PDB 8i0w Chain z Binding Site BS02

Receptor Information

>8i0w Chain z (length=80) Species: 9606 (Homo sapiens)

ARNAEKAMTALARFRQAQLEEPFLASECTELPKAEKWRRQIIGEISKKVA
QIQNAGLGEFRIRDLNDEINKLLREKGHWE

Ligand information

>8i0w Chain H (length=139)

aucgcuucucggccuuuuggcuaagaucaaguguaguaucuguucuuuua
auauguccucuaccgaggacaauauuaaggauuuuuggagcagggagcca
cgcaucgaccugguauugcaguaccuccaggaacggugc

...............................................<<<
<<<<<<<<<<....>>>>>>.>>>>>>>...............<......
><<<<<<.<<<<<.............>>>>>..>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8i0w Molecular basis for the activation of human spliceosome
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): A11 R14
• Binding residue (residue number reindexed from 1): A10 R13

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding

Biological Process

• GO:0000350 generation of catalytic spliceosome for second transesterification step
• GO:0000389 mRNA 3'-splice site recognition
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0000974 Prp19 complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005681 spliceosomal complex
• GO:0071006 U2-type catalytic step 1 spliceosome
• GO:0071013 catalytic step 2 spliceosome
• GO:0071014 post-mRNA release spliceosomal complex
• GO:0071020 post-spliceosomal complex

External links

• PDB: RCSB:8i0w, PDBe:8i0w, PDBj:8i0w
• PDBsum: 8i0w
• PubMed: 39068178
• UniProt: Q9ULR0|ISY1_HUMAN Pre-mRNA-splicing factor ISY1 homolog (Gene Name=ISY1)