Structure of PDB 7r72 Chain w Binding Site BS02

Receptor Information

>7r72 Chain w (length=189) Species: 1247190 (Saccharomyces cerevisiae BY4741)

TEEEQIEKDLQGLQEKQRLNVKRERRRKNEMKQKELQRMQMNMESLFNLK
TAEKTGILNDLAKGKKRMIFTMIKDKDSAADADDLESELNAMYSDYKTRR
SERDAKFRAKQARAITNLISKLKGQEGDHKLSSKARMIFNDPIFNNVEPF
DSDYDSEEEKNQTKKEKHSRDENLPDWFLEDAMNARPIK

Ligand information

>7r72 Chain G (length=19) Species: 1247190 (Saccharomyces cerevisiae BY4741)

LSRYVKWPEYVRVQRQKKI

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7r72 Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
• Resolution: 3.07 Å
• Binding residue (original residue number in PDB): E465 L466 M469 W698
• Binding residue (residue number reindexed from 1): E88 L89 M92 W177

Enzymatic activity

• Enzyme Commision number: 2.1.1.167: 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase.

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008168 methyltransferase activity
• GO:0008649 rRNA methyltransferase activity
• GO:0008650 rRNA (uridine-2'-O-)-methyltransferase activity
• GO:0016435 rRNA (guanine) methyltransferase activity
• GO:0070039 rRNA (guanosine-2'-O-)-methyltransferase activity

Biological Process

• GO:0000451 rRNA 2'-O-methylation
• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0001510 RNA methylation
• GO:0006364 rRNA processing
• GO:0031167 rRNA methylation
• GO:0032259 methylation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7r72, PDBe:7r72, PDBj:7r72
• PDBsum: 7r72
• PubMed: 36482249
• UniProt: P25582|SPB1_YEAST 27S pre-rRNA (guanosine(2922)-2'-O)-methyltransferase (Gene Name=SPB1)