Structure of PDB 6wdl Chain o Binding Site BS02

Receptor Information

>6wdl Chain o (length=116) Species: 83333 (Escherichia coli K-12)

DKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAA
STVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHG
RVQALADAAREAGLQF

Ligand information

>6wdl Chain 2 (length=120)

ugccuggcggccguagcgcgguggucccaccugaccccaugccgaacuca
gaagugaaacgccguagcgccgaugguaguguggggucuccccaugcgag
aguagggaacugccaggcaa

<<<<<<<<<<.....<<<<<<<<....<<<<<<<.............>>>
>..>>>...>>>>>>.>>.<<........<<<<<<<...>>>>>>>....
....>>...>>>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6wdl Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): K4 R15 R25 H29 R30 T31 P32 R33 H34 Y36 I40 V47 V54 E55 K63 Y64 N67 K68 Q98 H100 G101 R102
• Binding residue (residue number reindexed from 1): K3 R14 R24 H28 R29 T30 P31 R32 H33 Y35 I39 V46 V53 E54 K62 Y63 N66 K67 Q97 H99 G100 R101

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6wdl, PDBe:6wdl, PDBj:6wdl
• PDBsum: 6wdl
• PubMed: 32612237
• UniProt: P0C018|RL18_ECOLI Large ribosomal subunit protein uL18 (Gene Name=rplR)