Structure of PDB 8azw Chain g Binding Site BS02

Receptor Information

>8azw Chain g (length=209) Species: 4097 (Nicotiana tabacum)

GRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVH
LVSWEKENVSSEALEAARIACNKYMTKSAGKDAFHLRVRVHPFHVLRINK
MQTGMRGAFGKPQGVCARVAIGQVLLSVRCKDGNANHAQEALRRAKFKFP
GRQKIIVSRKWGFTKFSRTDYLKYKSENRIIPDGVNAKLLGCHGRLAARQ
PGRAFLEAV

Ligand information

>8azw Chain A (length=3196)

gcgaccccaggucaggcgggacuacccgcugaguuuaagcauaucaauaa
gcggaggaaaagaaacuuacaaggauuccccuaguaacggcgagcgaacc
gggaacagcccagccuuagaaucgggcggcuccgccguucgaauuguagu
cuggagaagcguccucagcggcggaccgggcccaaguccccuggaagggg
gcgccagagagggugagagccccguugugcccggacccugucgcaccacg
aggcgcugucuacgagucggguuguuugggaaugcagcccaaaucgggcg
gugaauuccguccaaggcuaaauacgggcgagagaccgauagcgaacaag
uaccgcgagggaaagaugaaaaggacuuugaaaagagagucaaagagugc
uugaaauugucgggagggaagcggaugggggccggcgaugcgccccgguc
ggauguggaacggugacgagccgguccgccgaucgacucggggcguggac
cagcguggauugggggggcggccaaagcccgggcuuucgauacgcccgug
gaacgccgucuccuugauugugguaggcagcgcgcgccuccggcgugcuu
cggcaucugcgcgcuccggacgcuggccugugggcuccccauucgacccg
ucuugaaacacggaccaaggagucugacaugugugcgagucaacgggcga
guaaacccguaaggcguaaggaagcugauuggugggaucccccugagggg
ugcaccgccgaccgaccuugaucuucugugaaggguucgagugugagcau
accugucgggacccgaaagauggugaacuaugccugagcggggcgaagcc
agaggaaacucugguggaggcccgcagcgauacugacgugcaaaucguuc
gucugacuuggguauaggggcgaaagacuaaucgaaccgucuaguagcug
guucccuccgaaguuucccucaggauagcuggagcucgcgugcgaguucu
aucggguaaagccaaugauuagaggcaucgggggcgcaacgcccucgacc
uauucucaaacuuuaaauagguaggacggugcggcugcuuuguugagccg
caccacggaaucaagagcuccaagugggccauuuuugguaagcagaacug
gcgaugcgggaugaaccggaagccggguuacggugccaaacugcgcgcua
accuagaucccacaaaggguguuggucgauuaagacagcgccgaaucaac
uagccccgaaaauggauggcgcuuaagcgcgcgaccuacacccggccguc
ggggcaagugccaggccccgaugaguaggagggcgcggcggucgcugcaa
aaccuugggcgcgagccugggcggagcggccgucggugcagaucuuggug
guaguagcaaauauucaaaugagaacuuugaaggccgaagaggggaaagg
uuccaugugaacggcacuugcacauggguuagucgauccuaagggucggg
ggaaccccgacagauagcgcguuucgcgcguacuccgaaagggaaucggg
uuaaaauuccugaaccgggacguggcgguugacggcaacguuaggaaguc
cggagacgucggcgggggccucgggaagaguuaucuuuucuguuuaacag
ccugcccacccuggaaacgacucagucggagguaggguccagcggcugga
agagcaccgcacgucgcgugguguccggugcgcccccggcggcccuugaa
aauccggaggaccgagugccguccacgcccggucguacucauaaccgcau
caggucuccaaggugaacagccucuggucgauggaacaauguaggcaagg
gaagucggcaaaauggauccguaacuucgggaaaaggauuggcucugagg
gcugggaacagccaacucagaacugguacggacaaggggaauccgacugu
uuaauuaaaacaaagcauugcgauggucccugcggauguuuacgcaaugu
gauuucugcccagugcucugaaugucaaagugaagaaauucaaccaagcg
cggguaaacggcgggaguaacuaugacucucuuaagguagccaaaugccu
cgucaucuaauuagugacgcgcaugaauggauuaacgagauucccacugu
cccugucuacuauccagcgaaaccacagccaagggaacgggcuuggcaga
aucagcggggaaagaagacccuguugagcuugacucuaguccgacuuugu
gaaaugacuugagaggguaguauaagugggagccggcgaaagugaaauac
cacuacuuuuaacguuauuuuacuuauuccgugaaucggaagcggggcac
ugccccucuuuuuggacccaaggcuugcuugcaggccgauccgggcggaa
gacauugucagguggggaguuuggcuggggcggcacaucuguuaaaagau
aacgcagguguccuaagaugagcucaacgagaacagaaaucucgugugga
acagaaggguaaaagcucguuugauucugauuuccaguacgaauacgaac
cgugaaagcguggccuaacgauccuuuagaccuucggaauucgaagcuag
aggugucagaaaaguuaccacagggauaacuggcuuguggcagccaagcg
uucauagcgacguugcuuuuugauccuucgaugucggcucuuccuaucau
ugugaagcagaauucaccaaguguuggauuguucacccaccaauagggaa
cgugagcuggguuuagaccgucgugagacagguuaguuuuacccuacuga
ugacagugucgcaauaguaauucaaccuaguacgagaggaaccguugauu
cgcacaauuggucaucgcgcuugguugaaaagccaguggcgcgaagcuac
cgugcgcuggauuaugacugaacgccucuaagucagaauccgggcuagaa
gcgacgcaugcgcccgccguccgcuugccgacccgcaguaggggccuuug
gcccccaagggcacgugucguuggcuaagucaucguggcggaagagccgc
gguggccgccuugaaguacaauuuccaucgagcggcggguagaauccuuu
gcagacgacuuaaauacgcgacgggguauuguaaguggcagaguggccuu
gcugccacgauccacugagauucagcccuuugucgcuccgauucgu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<.......<.....>......
>>>>>..[[[....<......<<<<<<<<<...>>>>>>>>>......>.
....<<<<<<<<.<<<.<<<<<<<.<<<<<<.<<...<<<<......>>>
>.((......<<<.....))>>>..>>.>>>>>>...>>>>>>>.....>
>>>>>>>.>>>.......<<<<<<<........>>>>>>>.....(((((
..<<<<<<)))))..]]]................................
..<<....>>...............<<<<....>>>>.............
.....................<<<<<<<<<<<<......<<<<<<<<<<<
<...<<<<<.<<<<.....>>>>.>>>>>....>>>>.>>>>>>>>.<.<
<<<<<..<<<<<<<<<<<<<<<.....<<<<<<<.........>>>>>.>
>...>>>>>>>>>>>>>>>.........<<<<<<<<<<...>>>..<<..
..>>....>>>>>>>.....>>>>>>.>....>>>.>>>>>>>>>..<<<
<........>>>><<<<<<.....<<<<<.<<<<<<..<<<...<<<...
.....>>>....>>>...<<....>>....<<<<<...<<<<....>>>>
....>>>>>.<<<<<<<.....<....>.....>>.>>>.>>....>>>>
>>.>>>>>...<<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<
<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>>>>>>
>>....>>>>>>>>>...<<.......>>......>>>>>>...>..>.>
>..<<<<<<....<.<<<....>>>.><<<<<<<<<<......<<.<<<<
<<<.....<<<....<<<....<<<....<<<<<<.....>>>>>>..>>
>....>>>..>.>>.....>>>>>...<<<<<<<<<.<......>>>>>>
>>>>....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>
>>....<<<......>>>...<<<<<<<...<<<(((.....<<<<<<..
.....<..........<<<.<<<<<<.<<<.......<..>...>>>.>>
>>>>>>>..........))).>....>>>>>>.>>>...>>>>>>><<<<
<<<<<.........>>>>>>>>>....>>>>>><.<<<<<<<<<<.....
..<<<.<<<<....>>>>>>>....>>>>>>>>>>..>.....>>>>>><
<.....<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<.
..<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<
<....>>>........<<<<<...>>>>>.....>>....>>...<<<<<
........>>>>>))<<<<.<<<<<<<<...<<<....>>>...<...<<
<<<<...<<<<.<<<<<<<.<<<<<.<<<<.......>>>>.><...<<<
<<.....<<<<<<....<<<<...>>>>....>>>>>>.....>>>>>..
..><<<<<<<.......>>>>>>>>>>>...>>>>>>>.>>>>.......
..>>>>>>...>......>>>>.>>>>>>>>>>>>..>>.....<<....
..>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<
.........<.....<<<<<....<<..>>.....>>>>><<.<<<<<<<
<<<<....>>>>>..>>>>>>.>>............<<<<<<<<<..<<<
.........>>>..<<<<<<<....<<<<....>>>>......>>>>>>>
...<<..<<<..<<<<<<.<..<<<.....<<<<....>>>>.....>>>
>>>>>..>>>>><<<<<<.......>>>>>>........<..........
.<<<<<.......>>>>>.....>.....>>....>>.>>>>>>>..>..
>>.>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....
[[[[[..{{{.{{......[[[[.{{[[[[..<<<<<.<<<.<<<...<<
<<<<<<<<.<<<<<<.........<<<<.<.<<<>>>............>
>>>>.>>>>>>..>>>>>>>>>>...<<<<<.....<<<<...<<<<<..
.>>>>>...............<<<<<<....>>>>>>..>>>>..>>>>>
......>>>.>>>..>>>>>...<<<.....>>>..<<<<<<........
...>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....
<<...(((>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.
<<<....>>>.>>))).......((((((..((((.>>>>>>))))))))
))..........]]]]..}}]]]].......<<.<.<.<<<<<<...<<.
......>>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<<<
.<<<<<......>>>>>...>>>.<<<.........>>>.....>>>>>.
.>...>>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]
]....<<<<<<<..<<<<.<.<<<<<.................>>>>>.>
<<<<<...<<<<....<<<<<<<<<......>>>>..>>>>>...>>>>.
.>>>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>>...
>>>>>>>....<<<<<<<<<.<<.<......<<.......<<<<<<...>
>>>>>....>>...........<<<...<<<<<<<<<<<......>>>>>
>>>>>>.>>>................>.>>.>>>>>>>>>.<<<<<.<..
...........>.....<<<<<<<<<.<<<....<<<<.<..<<<<<...
...>>>>>..>>>>>...>>>....>>>>..>>>>>...>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8azw Structure of the actively translating plant 80S ribosome at 2.2 angstrom resolution.
• Resolution: 2.14 Å
• Binding residue (original residue number in PDB): R3 R4 A6 R7 K13 N14 K15 P16 F22 R24 K30 D35 K39 K40 E63 A64 A67 K74 R90 H92 F94 R98 I99 N100 M102 G114 M115 R116 A118 F119 G120 Q133 R154 F157 K158 P160 K175 D193 G194 N196 K198
• Binding residue (residue number reindexed from 1): R2 R3 A5 R6 K12 N13 K14 P15 F21 R23 K29 D34 K38 K39 E62 A63 A66 K73 R89 H91 F93 R97 I98 N99 M101 G104 M105 R106 A108 F109 G110 Q123 R144 F147 K148 P150 K165 D183 G184 N186 K188

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8azw, PDBe:8azw, PDBj:8azw
• PDBsum: 8azw
• PubMed: 37156858
• UniProt: A0A1S3Z2D7