Structure of PDB 8b0x Chain f Binding Site BS02

Receptor Information

>8b0x Chain f (length=177) Species: 37762 (Escherichia coli B)

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

Ligand information

>8b0x Chain a (length=2748)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacagcugugu
gacugcguaccuuuuguauaaugggucagcgacuuauauucuguagcaag
guuaaccgaauaggggagccgaagggaaaccgagucuuaacugggcguua
aguugcaggguauagacccgaaacccggugaucuagccaugggcagguug
aagguuggguaacacuaacuggaggaccgaaccgacuaauguugaaaaau
uagcggaugacuuguggcugggggugaaaggccaaucaaaccgggagaua
gcugguucuccccgaaagcuauuuagguagcgccucgugaauucaucucc
ggggguagagcacuguuucggcaagggggucaucccgacuuaccaacccg
augcaaacugcgaauaccggagaauguuaucacgggagacacacggcggg
ugcuaacguccgucgugaagagggaaacaacccagaccgccagcuaaggu
cccaaagucaugguuaagugggaaacgaugugggaaggcccagacagcgu
cgagucggccugcgcggaagauguaacggggcuaaaccaugcaccgaagc
ugcggcagcgacgcgcguuguuggguaggggagcguucuguaagccugug
aaggugugcugugaggcaugcuggagguaucagaagugcgaaugcugaca
uaaguaacgauaaagcgggugaaaagcccgcucgccggaagaccaagggu
uccuguccaacguuaaucggggcagggugagucgaccccuaaggcgaggc
cgaaaggcguagucgaugggaaacagguuaauauuccuguacuugguguu
acugcgaaggggggacggagaaggcuauguuggccgggcgacgguugucc
cgguuuaagcguguaggcugguuuuccaggcaaauccggaaaaucaaggc
ugaggcgugaugacgaggcacuacggugcugaagcaacaaaugcccugcu
uccaggaaaagccucuaagcaucagguaacaucaaaucguaccccaaacc
gacacagguggucagguagagaauaccaaggcgcuugagagaacucgggu
gaaggaacuaggcaaaauggugccguaacuucgggagaaggcacgcugau
auguaggugaggucccucgcggauggagcugaaaucagucgaagauacca
gcuggcugcaacuguuuauuaaaaacacagcacugugcaaacacgaaagu
ggacguauacggugugacgccugcccggugccggaagguuaauugauggg
guuagcggcgcgaagcucuugaucgaagccccgguaaacggcggccguaa
cuauaacgguccuaagguagcgaaauuccuugucggguaaguuccgaccu
gcacgaauggcguaaugauggccaggcugucuccacccgagacucaguga
aauugaacucgcugugaagaugcaguguacccgcggcaagacggaaagac
cccgugaaccuuuacuauagcuugacacugaacauauguucuaacguugg
ccccugaucgggguugcggacagugucugguggguaguuugacuggggcg
gucuccuccuaaagaguaacggaggagcacgaagguuggcuaauccuggu
cggacaucaggagguuagugcaauggcauaagccagcuugacugcgagcg
ugacggcgcgagcaggugcgaaagcaggucauagugauccggugguucug
aauggaagggccaucgcucaacggauaaaagguacuccggggauaacagg
cugauaccgcccaagaguucauaucgacggcgguguuuggcaccucgaug
ucggcucaucacauccuggggcugaaguaggucccaaggguauggcuguu
cgccauuuaaagugguacgcgagcuggguuuagaacgucgugagacaguu
cggucccuaucugccgugggcgcuggagaacugaggggggcugcuccuag
uacgagaggaccggaguggacgcaucacugguguucggguugucaugcca
auggcacugcccgguagcuaaaugcggaagagauaagugcugaaagcauc
uaagcacgaaacuugccccgagaugaguucucccugacuccuugagaguc
cugaaggaacguugaagacgacgacguugauaggccggguguguaagcgc
agcgaugcguugagcuaaccgguacuaaugaaccgugaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<..>>>>>
..>>>>...<............>.<<<<<<....<<<<<<<<<<<<<..<
<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>....
.>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>
>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.....
.>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<<<<<
<<.....<<<...<<<.<<<<...<<.<<<<.....>>>>..>>...>>>
>.>>>..>>.>.....>>>>>>.>>>...>>>>>>>.>...<<<<<<<<<
<......>>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<
(((.....<<<<<<...........<<..<<<<<..<<........<..>
....>>..>>>>>.>>...........))).....>>>>>>.>>>...>>
>>>>><<<<<<<<..>>>>>>>>....>>>>>><.<<<<<...<<<....
..<<<<<<<<....>>>>>>>>...>>>..>>>>>..>.....>>>>>><
......<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<..
.<<<<<<...<<.....>>.>>>>>>....((<<<.<<<...<<<...<<
<....>>>...>>>...>>>..<<<<<........>>>>>))<<<<<<<<
<<<<<...<<<<....<<<<..<<<..<<<<<<<<<<<....(((...>>
>>>><<..<<<<...<<<<<<<<<<<<.<<.....>>>>>>>>>>..>>>
>...>>>>...>><..<<<<<....>>>>>...>>>>>>...>>>...>>
>>.........>>>>...>>....>>>>>>>>>>>.>>>..>>.....<<
......>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<
<.<<...............<<<<<...............>>>>><<<<<<
.....<<<...<<<<.....>>>>...>>>...>>>>>>...........
.<<<<<<<<<..<<<.........>>>..<<<<<<<.....<<<....>>
>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<<...<...<<<
<<..<<<.>>>...>>>>>..>....>>>>>>>>>..>>>>><<<<<<..
.....>>>>>>........<...........<<<<<<.....>>>>>>..
...>....>>>>>..>>.>>>>>>>.....>>.>>>>>>>>.>>>>>>..
...>.....<<<<<........>>>>>..<<<<<<<<.(((.[[......
<<<<.[[<<<<..<<<<<.<<<.<<<<<.<<<<<..>>>>>...<<<.<<
<<<<.....>>>>>>>>>....>>>>>.>>>..>>>>>..<<<<.....>
>>><<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<.
......>>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<
<....>>>>..>>>>.<<<....>>>.>>))).......<<<<<<<.((.
.>>>>..))>>>>>>>.............>>>>..]]>>>>.......<<
<<...<<<<<<...<<.......>>...>>>>>>....>>>.>.......
<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<....
.>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>
>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<...
............>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))).
>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<.........
..>>>>>....>>>.>>>>.......>>>>>>>..<<<<<.....>>>>>
<....<<..<<<<<.......>>>>>....<<<<<<<<..<<....<<<<
<....>>>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8b0x The translating bacterial ribosome at 1.55 angstrom resolution generated by cryo-EM imaging services.
• Resolution: 1.55 Å
• Binding residue (original residue number in PDB): K33 N37 M38 G39 G41 T68 A70 R71 F77 I79 I85 K88 S121 D123 R125 S129 G131 R133 G151 L152 D153 T155
• Binding residue (residue number reindexed from 1): K32 N36 M37 G38 G40 T67 A69 R70 F76 I78 I84 K87 S120 D122 R124 S128 G130 R132 G150 L151 D152 T154

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8b0x, PDBe:8b0x, PDBj:8b0x
• PDBsum: 8b0x
• PubMed: 36841832
• UniProt: P62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)