Structure of PDB 5czp Chain XY Binding Site BS02

Receptor Information

>5czp Chain XY (length=357) Species: 83333 (Escherichia coli K-12)

PVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERAQA
LGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAELDA
LEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRW
AESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKS
PFDSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRTSGAGGQHVN
RTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYEVEMQKKNA
EKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVETRNTQAVLDGSLDQ
FIEASLK

Ligand information

>5czp Chain XA (length=1504)

uuggagaguuugauccuggcucagggugaacgcuggcggcgugccuaaga
caugcaagucgugcgggccgcgggguacuccguggucagcggcggacggg
ugaguaacgcgugggugaccuacccggaagagggggacaacccggggaaa
cucgggcuaaucccccauguggacccgccccuugggguguguccaaaggg
cuuugcccgcuuccggaugggcccgcgucccaucagcuaguugguggggu
aauggcccaccaaggcgacgacggguagccggucugagaggauggccggc
cacaggggcacugagacacgggccccacuccuacgggaggcagcaguuag
gaaucuuccgcaaugggcgcaagccugacggagcgacgccgcuuggagga
agaagcccuucgggguguaaacuccugaacccgggacgaaacccccgacg
aggggacugacgguaccgggguaauagcgccggccaacuccgugccagca
gccgcgguaauacggagggcgcgagcguuacccggauucacugggcguaa
agggcguguaggcggccuggggcgucccaugugaaagaccacggcucaac
cgugggggagcgugggauacgcucaggcuagacggugggagaggguggug
gaauucccggaguagcggugaaaugcgcagauaccgggaggaacgccgau
ggcgaaggcagccaccugguccacccgugacgcugaggcgcgaaagcgug
gggagcaaaccggauuagauacccggguaguccacgcccuaaacgaugcg
cgcuaggucucugggucuccugggggccgaagcuaacgcguuaagcgcgc
cgccuggggaguacggccgcaaggcugaaacucaaaggaauugacggggg
cccgcacaagcgguggagcaugugguuuaauucgaagcaacgcgaagaac
cuuaccaggccuugacaugcuagggaacccgggugaaagccuggggugcc
ccgcgaggggagcccuagcacaggugcugcauggccgucgucagcucgug
ccgugagguguuggguuaagucccgcaacgagcgcaacccccgccguuag
uugccagcgguucggccgggcacucuaacgggacugcccgcgaaagcggg
aggaaggaggggacgacgucuggucagcauggcccuuacggccugggcga
cacacgugcuacaaugcccacuacaaagcgaugccacccggcaacgggga
gcuaaucgcaaaaaggugggcccaguucggauuggggucugcaacccgac
cccaugaagccggaaucgcuaguaaucgcggaucagccaugccgcgguga
auacguucccgggccuuguacacaccgcccgucacgccaugggagcgggc
ucuacccgaagucgccgggagccuacgggcaggcgccgaggguagggccc
gugacuggggcgaagucguaacaagguagcuguaccggaaggugcggcug
gauc

.....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<..<<
<.<<<..<<<..<<..<<<<<<<<....>>>>>>>>..>>>>>......<
<.......<<<<<<<<..<<...<<<<<<<.<<<<<....<<<<<.....
.>>>>>.....>>>>>...<<<<<.<<<<<....>>>>>.>>>>>..<<<
<...>>>>.>>>>>>>..>>>>>>>>>><<<....<<<..<<<<<<<<..
.....>>>>>>>>>>>......>>>..<<<<<<<<....>>>>...>>>>
.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>>>>>.
.......<<<....<<<<....>>>>..>>>..>>.>>>>>>..<<<<..
....<<<<....>>>>.....>>>>....<<<<<......<.<<<<....
.>>>>..>.......>>>>>......<<<<<(((...<<<<<.....<<.
)))>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((..
...<<<<...<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....>
>>>>>....>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<.
...<<<<<<<.<..<<<......>>>.....>.>>>>>>>..........
.<<....>>.>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<
<...<<...<<<<.........>>>>...>>>>>>>>..........<<<
<<<..<<<<<<<<<<...>>>>>>>>>>...<<..))>>.....>>>>>>
.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<<.<<<
<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>......
....<<<<<<<......<<<<<<<<..<<<<<<<....>>>>>>>....<
<<....>>>...>>>>>>>>.<<<.<<<..<<<<<<.......<<<<<<<
<<....>>>..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<<
..<<<..<<<.....>>>>>>...>>>>>>>.....<<<<<....>>>>>
........>>>>.........>>>...>>>>>>>>>...>>>>>>>...>
>.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<....>>>>..
>>....>>>.....>>>>>>>......<....<<<<<<<........>>>
>>>>....>.....>>>>>>....<<<<<<<..........>>>>>>>..
....>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<
<<<<<<<...<.<<<<....<<<....>>>.>>>>.>..>>>>>>>>>>>
>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>.
....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5czp Uniformity of Peptide Release Is Maintained by Methylation of Release Factors.
• Resolution: 3.29996 Å
• Binding residue (original residue number in PDB): P6 G135 S136 R154 R200 S209 R212 R213 W319 Q322 D329 D330 R332 R343
• Binding residue (residue number reindexed from 1): P1 G130 S131 R149 R195 S204 R207 R208 W314 Q317 D324 D325 R327 R338

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0016149 translation release factor activity, codon specific

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination
• GO:0075523 viral translational frameshifting

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5czp, PDBe:5czp, PDBj:5czp
• PDBsum: 5czp
• PubMed: 27681416
• UniProt: P07012|RF2_ECOLI Peptide chain release factor RF2 (Gene Name=prfB)