Structure of PDB 6hcf Chain W3 Binding Site BS02

Receptor Information

>6hcf Chain W3 (length=106) Species: 9986 (Oryctolagus cuniculus)

MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQIN
WTVLYRRKHKKGQQRAITGASLADIMAKRNQKPEVRKAQREQAIRAAKEA
KKAKQA

Ligand information

>6hcf Chain 52 (length=3634)

cgcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguc
agcggaggaaaagaaacuaaccaggauucccucaguaacggcgagugaac
agggaagagcccagcgccgaauccccgccccgcggcggggcgcgggaaau
guggcguacggaagacccacuccccggcgccggggggcccaaguccuucu
gaucgaggcccagcccguggacggugugaggccgguagcggcccccggcg
cgccgggcccgggucuucccggagucggguugcuugggaaugcagcccaa
agcgggugguaaacuccaucuaaggcuaaauaccggcacgagaccgauag
ucaacaaguaccguaagggaaaguugaaaagaacuuugaagagagaguuc
aagagggcgugaaaccguuaagagguaaacgggugggguccgcgcagucc
gcccggaggauucaacccggcggcgcgguccggccgugccggcggggcgg
aucucccgcucccgucggcgggggaccgccccccggccggcgaccggccg
ccgccgggcgcauuuccaccgcggcggugcgccgcgaccggcuccgggac
ggcugggaaggccccggcggggaagguggcccgggggccccggguguuac
agccccgccccggcagcagcagcucgccgaaucccggggccgagggagcg
agacccgugcgccgcgcucuccccccucgggggggccgggccaccccucc
cacggcgcgaccgcucucccacccggggggcggggcggacuguccccagu
gcgccccgggcgggucgcgccgucgggcccgggggagguucucucggggc
cacgcgucccccgcgagcgcacggggucggcggcgaugucggcuacccac
ccgacccgucuugaaacacggaccaaggagucuaacacgugcgcgagucg
ggggcuacgaaagccgccguggcgcaaugaaggugaaggccggcggcgcg
ccgccggccgaggugggaucccgaggccuuccaguccgccgagggcgcac
caccggcccgucucgcccgccgcgccggggagguggagcacgagcgcacg
uguuaggacccgaaagauggugaacuaugccugggcagggcgaagccaga
ggaaacucugguggagguccguagcgguccugacgugcgaaucggucguc
cgaccuggguauaggggcgaaagacuaaucgaaccaucuaguagcugguu
cccuccaaaguuucccucaggauagcuggcgcucuccgcaguuuuauccg
guaaagcgaaugauuagaggucuuggggccgaaacgaucucaaccuauuc
ucaaacuuuaaauggguaagaagcccggcucgcugguguggagccgggcg
uggaaugcgagugccuagugggccacuuuugguaagcagaacuggcgcug
cgggaugaaccgaacgccggguuaaggcgcccgaugccgacgcucaucag
accccagaaaagguguugguugauauagacagcaggaugguggccaugga
agucggaauccgcuaaggaguguguaacaacucaccugccgaaucaacua
gcccugaaaauggauggcgcuggagcgucgggcccauacccggccgucgc
uggcagucggcagagcgagaaagagaguggcgcggacgcuacgccgcgac
gaguaggagggccgcugcggugagccuugaagccuagggcgcgggcccgg
guggagccgccgcaggugcagaucuuggugguaguagcaaauauucaaac
gagaacuuugaaggccgaaguggagaaggguuccaugugaacagcaguug
aacaugggucagucgguccugagagaugggcgagcgccguuccgaaggga
cgggcgauggccuccguugcccucagccgaucgaaagggagucggguuca
gauccccgaauccggaguggcggagaugggcgccgcgaggcguccagugc
gguaacgcgaccgaucccggagaagccggcgggagccccggggagaguuc
ucuuuucuuugugaagggcagggcgcccuggaauggguucgccccgagag
aggggcccgugccuuggaaagcgucgcgguuccggcggcguccggugagc
ucucgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccgg
gccguacccauauccgcagcaggucuccaaggugaacagccucuggcaug
uuggaacaauguagguaagggaagucggcaagccggauccguaacuucgg
gauaaggauuggcucuaagggcuggcagccgacuuagaacuggugcggac
caggggaauccgacuguuuaauuaaaacaaagcaucgcgaaggcccgcgg
cggguguugacgcgaugugauuucugcccagugcucugaaugucaaagug
aagaaauucaaugaagcgcggguaaacggcgggaguaacuaugacucucu
uaagguagccaaaugccucgucaucuaauuagugacgcgcaugaauggau
gaacgagauucccacugucccuaccuacuauccagcgaaaccacagccaa
gggaacgggcuuggcggaaucagcggggaaagaagacccuguugagcuug
acucuagucuggcacggugaagagacaugagagguguagaauaaguggga
ggcccgggccgccggugaaauaccacuacucugaucguuuuuucacugac
ccggugaggcgggggggcgagccgggcucucgcuucuggcgccaagcgcc
cggccggccgggcgcgacccgcuccggggacagugccagguggggaguuu
gacuggggcgguacaccugucaaacgguaacgcagguguccuaaggcgag
cucagggaggacagaaaccucccguggagcagaagggcaaaagcucgcuu
gaucuugauuuucaguacgaauacagaccgugaaagcggggccucacgau
ccuucugaccuuuuggguuuuaagcaggaggugucagaaaaguuaccaca
gggauaacuggcuuguggcggccaagcguucauagcgacgucgcuuuuug
auccuucgaugucggcucuuccuaucauugugaagcagaauucaccaagc
guuggauuguucacccacuaauagggaacgugagcuggguuuagaccguc
gugagacagguuaguuuuacccuacugaugauguguuguugccaugguaa
uccugcucaguacgagaggaaccgcagguucagacauuugguguaugugc
uuggcugaggagccaauggggcgaagcuaccaucugugggauuaugacug
aacgccucuaagucagaaucccgcccaggcggaacgauagcgccggagcc
ucgguuggccucggauagccggucccccgccgggguccggugcggagagc
ccuucgucccgggaaacggggcggccggaaagggggccgcccucucgccc
gucacgcaccgcacguucguggggaaccuggcgcuaaaccauucguagac
gaccugcuucugggucgggguuucguacguagcagagcagcugcugcgau
cuauugaaagucagcccucgacacaaggguuugu

.........................<<<<<<.....<......>......
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>>>>>>..[[[..<<<<<...<<<<<<<<<<.....>>>>>>.>>>>...
..>>>>>..<<<<<<<<<....<<<<<<<<..<<<<<<<<....<<<<..
....>>>>.....((......<<<.....))>>>...>.>>>>>>>...>
>>>>>>>...>>>>>>>>>.......<<<<<<<........>>>>>>>..
....((((..<<<<<<))))...]]]........................
..........<<....>>...............<<<......>>>.....
.............................<<<<<<<<..<<<.<....<<
<<<<<<.<<<.....<<<<<<<<<<.....<<<<<<.<<<<<.<<<<<<<
.<<<<<<<.<.......>>>>>>>>>>>>>>>>>>>>>>>>...>>..>>
>>>>>>>>......>>>.>>>.>>>>>...<<<<<<<.<<<<<<<<<<.<
<<<.........<..<<<<<<<......<<<<<<<...>>>>>>>.....
..>>>>>>>...>............>>>>...>>>>>>>>>>.....<<.
......>>.........<....<<<<<..>>>>>....<.<<..<<<.<<
<<<<<<<<<<<<<<<...<<.<<..>>>>.<<<<<<<...<<....>>..
.>>>>>>>>>>>.>>>>>>>>>>.>>>...>>>...<<....>>..>>.>
................>.........>>.>>>>>....>>>>...>>>>>
>>>..<<<<........>>>><<<<<<....<<<<<<<<<<<<<..<<<.
..<<<.......>>>.....>>>...<<....>>...<<<<<<<<<<..>
>>>>>>>>>..<<<<<...<<<..<<<...(((.[[.>>>..>>>....>
>>>>.<<<<<<<<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>
>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<..<<<
<....>>>>..>.....>>>...<<<..<..<<........>>.>.>>>.
...>>>>>>>>>...<<.......>>......>>>>>>......>.>>..
<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<<<<<<<.
...<<<....<<<..<.<<<..<<<<.<.........>.>>>>>>>.>..
>>>...>>>....>>>>>>...<<<<<<<<<.<......>>>>>>>>>>.
...>>>>>>>>>>>>.>>>..<<<<.<<<<<.....>>>>>.>>>>....
<<<......>>>...<<<<<<<...<<<(((.......<<<<<.....<<
..........<<<.<<<<<<<<<........<..<........<<.....
....>>...............<<......>>.....>.>....>>>>>>>
>>>>>..........))).>>...>>>>>..>>>...>>>>>>><<<<<.
.<<<....................]].)))............>>>.>>>>
>....>>>>>>.<<<<<<<<<..<.......<<<<.<<<<....>>>>>>
>>....>.>>>>>>>.>>.....>>>>>><<<....<<.....<<<<<<.
......>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<.....>>
.>>>>>>......<<<<<<...<<....<<<....>>>..........<.
.<<<<<.<<<...>>>.>>>>>.>......>>....>>>..<<<<<....
....>>>>>..<<<<....<<<.....<<<<<<<....>>>>>>>...<<
<....>>>......<<<<......<<<<<<<<<<<<<<<<<<<<<<...{
{{...>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>.....
>>>>>>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>>.>.>>
>>>>>>>>>>............>>>>................>>>.>>>>
.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>...
.<<<<.....<<<<<<<.<<.........<.....<<<<<....<<..>>
.....>>>>><<.<<<<<<<<<<<.>>>>>..>>>>>>.>>.........
...<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<....
.>>>>......>>>>>>>...<<..<<<..<<<<<<....<<<....<<<
<<....>>>>.>...>>>.>>>>..>>>>><<<<<<.......>>>>>>.
.......<...........<<<<<.......>>>>>.....>.....>>.
...>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>...>>>....<<<<
..........>>>>.....[[[[..{{{.{{......[[[[.{..[[[..
<<<<..<<<<<<<<......<<.<.<<<.<<<<..........<<<<<..
<<<<..>>>>............>>>>>.>>>>.>>.>>..>>.......<
<<<<.....<<<<..<<.<<.......>>>>..............<<<<<
<<<<..>>>>>>>>>..>>>>..>>>>>.....>>>>>>>>...>>>>..
..<<.....>>...<<<<<<...........>>>>>>..<<...<<<<<<
<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>>>>>>.
..<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>>)))].....
.((((((..(((..>>>>>>.)))))))))..........]]]....}]]
]].......<<..<<.<<<<<<...<<.......>>...>>>>>>...>>
.>.>.........<<<.<.<<<...<...<<<<<......>>>>>.....
>.<<<.........>>>......>>>...>...>>>.......<<<<<<<
....>>>.>>>>....}}..}}}.]]]]......<<<<<<....<<<..<
.<<<<<.................>>>>>.><<<<....<<<<....<<.<
<<<<<.}}}..>>>>..>>.>>...>>>>...>>>><<<<....<<<<..
...........>>>>....>>>>.>>>.....>>>>>>.<<<<<<<..<<
<.....<<<........<<<<<<..<<<<.>>>>>.>>>>>.......>>
>...<<<..<<<<....<<<<<<<<<........>>>>>>.>>>...>>>
>..>>>...............>>>..>.>>>>>>.<<<<<.<........
.....>....<<.<<<<<<<.<<.....<<<<.<..<<<<<.>>>>>..>
>>>>....>>....>>>>.>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6hcf ZNF598 Is a Quality Control Sensor of Collided Ribosomes.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): H17 R19 K35 S38 N50 W51 R56 K61
• Binding residue (residue number reindexed from 1): H17 R19 K35 S38 N50 W51 R56 K61

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0010458 exit from mitosis
• GO:0021554 optic nerve development
• GO:0031290 retinal ganglion cell axon guidance
• GO:0060041 retina development in camera-type eye

Cellular Component

• GO:0005737 cytoplasm
• GO:0005783 endoplasmic reticulum
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6hcf, PDBe:6hcf, PDBj:6hcf
• PDBsum: 6hcf
• PubMed: 30293783
• UniProt: G1SE28|RL24_RABIT Large ribosomal subunit protein eL24 (Gene Name=RPL24)