Structure of PDB 5wfs Chain V Binding Site BS02

Receptor Information

>5wfs Chain V (length=92) Species: 562 (Escherichia coli)

FTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQ
AKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVR

Ligand information

>5wfs Chain B (length=120)

ugccuggcggccguagcgcgguggucccaccugaccccaugccgaacuca
gaagugaaacgccguagcgccgaugguaguguggggucuccccaugcgag
aguagggaacugccaggcaa

<<<<<<<<<<.....<<<<<<<<....<<<<<<<.............>>>
>..>>>...>>>>>>.>>.<<.......<<<<<<<<...>>>>>>>>...
....>>...>>>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5wfs Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): R9 Q12 G13 K14 S17 R18 R19 R21 I29 Y31 P37 Q75 Q78 H88
• Binding residue (residue number reindexed from 1): R8 Q11 G12 K13 S16 R17 R18 R20 I28 Y30 P36 Q74 Q77 H87

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0009314 response to radiation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5wfs, PDBe:5wfs, PDBj:5wfs
• PDBsum: 5wfs
• PubMed: 29733411
• UniProt: P68919|RL25_ECOLI Large ribosomal subunit protein bL25 (Gene Name=rplY)