Structure of PDB 2xcr Chain U Binding Site BS02

Receptor Information

>2xcr Chain U (length=717) Species: 158879 (Staphylococcus aureus subsp. aureus N315)

KLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSRTQAILPLRGKILNVEK
ARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTDADVDGAHI
RTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQ
TFEMLMGDVVENRRQFIEDNAVYANLDFAELPQSRINERNITSEMRESFL
DYAMSVIVARALPDVRDGLKPVHRRILYGLNEQGMTPDKSYKKSARIVGD
VMGKYHPHGDSSIYEAMVRMAQDFSYRYPLVDGQGNFGSMDGDGAAAMRF
TEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVLPARFPNLLANGAS
GIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDFPTAGL
ILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARM
IEKIAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYK
QTPLQTSFGVNMIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRK
AKDRAHILEGLRIALDHIDEIISTIRESDTDKVAMESLQQRFKLSEKQAQ
AILDMRLRRLTGLERDKIEAEYNELLNYISELETILADEEVLLQLVRDEL
TEIRDRFGDDRRTEIQL

Ligand information

>2xcr Chain W (length=18)

gccgtagggccctacggc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2xcr Type Iia Topoisomerase Inhibition by a New Class of Antibacterial Agents.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): G436 D437 S438 R458 D508 K1043 V1045 H1079 H1081 S1085 R1092 S1173 I1175
• Binding residue (residue number reindexed from 1): G20 D21 S22 R42 D92 K270 V272 H306 H308 S312 R319 S400 I402

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

External links

• PDB: RCSB:2xcr, PDBe:2xcr, PDBj:2xcr
• PDBsum: 2xcr
• PubMed: 20686482
• UniProt: P66937|GYRB_STAAN DNA gyrase subunit B (Gene Name=gyrB);
  Q99XG5|GYRA_STAAN DNA gyrase subunit A (Gene Name=gyrA)