Structure of PDB 6ke6 Chain SR Binding Site BS02

Receptor Information

>6ke6 Chain SR (length=125) Species: 559292 (Saccharomyces cerevisiae S288C)

AVPSVQTFGKKKSATAVAHVKAGKGLIKVNGSPITLVEPEILRFKVYEPL
LLVGLDKFSNIDIRVRVTGGGHVSQVYAIRQAIAKGLVAYHQKYVDEQSK
NELKKAFTSYDRTLLIADSRRPEPK

Ligand information

>6ke6 Chain SA (length=1310)

agauaguuaucugguugauccugccaguagucauaugcuugucucagcca
ugcaugucuaaguauaagcaauuuauacagugaaacugcgaaggcucauu
aaaucaguuaucguuuauuugauaguuccaugguauaacugugguaauuc
uagagcuaauacaugcuaaucucgacccuuuggaagagauguauuuauua
gaucaaugucuucggacucuuugaugaaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaaggca
gcaggccaaauuacccaauccuaauucagggagguagugacaauaaauaa
cgauacagggcccauucgggucuuguaauuggaaugaguacaauguaaau
accuuaacgaggaacaauuggagggcaagucuggugccagcagccgcggu
aauuccagcuccaauagcguauauuaaaguuguugcagaagcucguaguu
gaacuggcccgguuggccgguccggauuuccaacggggccuugauuaaua
gggacggucgggggcaucaguauucaauugucagaggugaaauucuugga
uuuauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauu
aaucaagaacgaccauaaacuaugccgacuagggaucgggugguguuuuu
uuaaugacccacucggcaccuuacgagaacuggggggaguauggucgcaa
ggcugaaacuuaaaggaauugacggaagggcaccaccaggaguggagccu
gcggcucaacacggggaaacucaccagguccagacacaauaaggauugac
auucuugauuuugugggugguggugcauugaugcccuuguucugggccac
gcgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucuu
gguaaucuugugaaacuccgucgugcuggggauauucaacgaggaauucc
uaguaagcgcaagucaucagcuugcguugauuacgucccugcccuuugua
cacaccgcccgucgcuaguaccgauugaauggcuuagugaggccucagga
ucugcuuagagaagggggcaacuccaucucagagcggagaauuuggacaa
acuuggucauuuagaggaacuaaaagucguaacaagguuuccguagguga
accugcggaa

...........................<<<<.<<<<<<...<....<<<.
<<...<<....<<....<<..........>>...>>.>>.....<<<...
....<<<..<<..<<....<<<..........<....<<<.<<<.....>
>>.>>.>....>.......<<<<............>>>>.....<<<<<.
..<<<<.<........>...>>>>...>>>>>.........<<<<..<<<
.....>>>.>>>>....>>>...>>>>..>>>.<.<....<<<....<<<
<<<<<.......>>>>>>>>>>>......>.>.....<<...........
....>>..>>>.<<.<<<..........>>>.>>...<<....>>.....
.>>>>>......<<<....<<<.....>>>..>>>.........>.....
<<<<<<<.<<<<<....>>>>>.>>>.>>>>......<<..<........
...>..>>.........<<<<<<.......<<<....>>>..........
........>>>>>>..>>>>>>>>>>..............<.<<...<<<
.<<..<<<<<.<<<<<..<.......>..>>>>>.>>>>>....>>....
<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>.....
.>.>>>>>>..>>.......<<....>>...>>>>..>.>>>>.>>>...
>>>...>>.>...............<<...<<<..<<<<<<<<.<<<...
.....>>>>>>>>>>>..>>>..>>.........................
..<<.........>>.....<<<<<<<<<<<<..<<.<<<<<<..<<<.<
<<<.......................<<......<<<<<...........
..........>>>>>......<<<........>>>.....>>.......>
>>>.>>>.....<<<<<<<............<<<..<<<<....>>>>..
>>>............>>>>>>>.......................>>>>>
>....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>....
........>>>>>..<<<<.<<.....<<<<<<<.<<<..<.<<......
<<<<<<<...<<.<<<<....>>>>.>>...>>>>>>>......>>.>..
>>>.>>>>>>>....>>.>>>>................<<<<<<<<<...
.>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6ke6 3.4 angstrom cryo-EM structure of yeast 90S small subunit preribosome
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): K14 S15 G71 G72 G73 V75 S76 P124 E125 K127
• Binding residue (residue number reindexed from 1): K12 S13 G69 G70 G71 V73 S74 P122 E123 K125

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation
• GO:0006413 translational initiation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6ke6, PDBe:6ke6, PDBj:6ke6
• PDBsum: 6ke6
• UniProt: P0CX51|RS16A_YEAST Small ribosomal subunit protein uS9A (Gene Name=RPS16A)