Structure of PDB 6lqp Chain RQ Binding Site BS02

Receptor Information

>6lqp Chain RQ (length=226) Species: 559292 (Saccharomyces cerevisiae S288C)

IDDRQVIENANLLKGKSSTYEVPLPQRIQQRHDRKAAYEISRQEVSKWND
IVQQNRRADHLIFPLNKPTEHNHASAFTRTQDVPQTELQEKVDQVLQESN
LANPEKDSKFEELPEEMRKRTTEMRLMRELMFREERKARRLKKIKSKTYR
KILQNVIINEKVNKKNLKYQSSAVPFPFENREQYERSLRMPIGQEWTSRA
SHQELIKPRIMTKPGQVIDPLKAPFK

Ligand information

>6lqp Chain SA (length=1325)

aagauaguuaucugguugauccugccaguagucauaugcuugucucagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaaggcucau
uaaaucaguuaucguuuauuugauaguuccaugguauaacugugguaauu
cuagagcuaauacaugcuaaucucgacccuuuggaagagauguauuuauu
agaucaaugucuucggacucuuugaugaaauaacuuuucgaaucgcaugg
ccuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgau
gguaggauaguggccuaccaugguuucaacggguaacggggaauaagggu
ucgauuccggagagggagccugagaaacggcuaccacauccaaggaaggc
agcaggccaaauuacccaauccuaauucagggagguagugacaauaaaua
acgauacagggcccauucgggucuuguaauuggaaugaguacaauguaaa
uaccuuaacgaggaacaauuggagggcaagucuggugccagcagccgcgg
uaauuccagcuccaauagcguauauuaaaguuguugcagaagcucguagu
ugaacuggcccgguuggccgguccggauuuccaacggggccuugguucuc
uaggacugauuaauagggacggucgggggcaucaguauucaauugucaga
ggugaaauucuuggauuuauugaagacuaacuacugcgaaagcauuugcc
aaggacguuuucauuaaucaagaacgaccauaaacuaugccgacuaggga
ucgggugguguuuuuuuaaugacccacucggcaccuuacgagaacugggg
ggaguauggucgcaaggcugaaacuuaaaggaauugacggaagggcacca
ccaggaguggagccugcggcucaacacggggaaacucaccagguccagac
acaauaaggauugacauucuugauuuugugggugguggugcauugaugcc
cuuguucugggccacgcgcgcuacacugacggagccagcgagucuaaccu
uggccgagaggucuugguaaucuugugaaacuccgucgugcuggggauau
ucaacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacg
ucccugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuu
agugaggccucaggaucugcuuagagaagggggcaacuccaucucagagc
ggagaauuuggacaaacuuggucauuuagaggaacuaaaagucguaacaa
gguuuccguaggugaaccugcggaa

............................<<<<.<<<<<<........<<<
.<<...<<....<<....<<..........>>...>>.>>.....<<<..
.....<<<..<<..<<....<<<..........<.....<<.<<......
.>>.>>......>.......<<<<............>>>>.....<<<<<
...<<<<..............>>>>...>>>>>.........<<<<...<
<.....>>..>>>>....>>>...>>>>..>>>.<<<....<<<....<<
<<<<<<.......>>>>>>>>>>>......>>>.....<<..........
.....>>..>>>.<<.<<<..........>>>.>>.<.<<....>>.>..
..>>>>>......<<<....<<<.....>>>..>>>..............
.<<<<<<<.<<<<<....>>>>>.>>>.>>>>......<<..<.......
....>..>>.........<<<<<<.......<<<....>>>.........
.........>>>>>>..>>>>>>>>>>.........<....<.<<...<<
<.<<..<<<<<.<<<<<..<.......>..>>>>>.>>>>>....<....
.>.......>>....<<<<<<.<<...<<<<..<<..<<<<<<.<...<<
<......>>>......>.>>>>>>..>>.......<<....>>...>>>>
...>>>>>.>>>...>>>...>>.>...............<<...<<<..
<<<<<<<<.<<<........>>>>>>>>>>>..>>>..>>......>...
.................<<.........>>.....<<<<<<<<<<<<..<
<.<<<<<<..<<<.<<<<.......................<<.<....<
<<<<........<.....>......>>>>>......<<<........>>>
...>.>>.......>>>>.>>>.....<<<<<<<............<<<.
.<<<<....>>>>..>>>............>>>>>>>.............
..........>>>>>>....<<<<<<<<.......>>>>>>>>......>
>...>>>>>>>............>>>>>..<<<<.<<.....<<<<<<<.
<<<..<.<<......<<<<<<<...<<.<<<......>>>.>>...>>>>
>>>......>>.>..>>>.>>>>>>>....>>.>>>>.............
...<<<<<<<<<....>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6lqp Cryo-EM structure of 90 S small ribosomal subunit precursors in transition states.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): Q303 R306 R307 F849
• Binding residue (residue number reindexed from 1): Q53 R56 R57 F176

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding
• GO:0005524 ATP binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6lqp, PDBe:6lqp, PDBj:6lqp
• PDBsum: 6lqp
• PubMed: 32943522
• UniProt: Q04500|UTP14_YEAST U3 small nucleolar RNA-associated protein 14 (Gene Name=UTP14)