Structure of PDB 6ke6 Chain RQ Binding Site BS02

Receptor Information

>6ke6 Chain RQ (length=226) Species: 559292 (Saccharomyces cerevisiae S288C)

IDDRQVIENANLLKGKSSTYEVPLPQRIQQRHDRKAAYEISRQEVSKWND
IVQQNRRADHLIFPLNKPTEHNHASAFTRTQDVPQTELQEKVDQVLQESN
LANPEKDSKFEELPEEMRKRTTEMRLMRELMFREERKARRLKKIKSKTYR
KILQNVIINEKVNKKNLKYQSSAVPFPFENREQYERSLRMPIGQEWTSRA
SHQELIKPRIMTKPGQVIDPLKAPFK

Ligand information

>6ke6 Chain SA (length=1310)

agauaguuaucugguugauccugccaguagucauaugcuugucucagcca
ugcaugucuaaguauaagcaauuuauacagugaaacugcgaaggcucauu
aaaucaguuaucguuuauuugauaguuccaugguauaacugugguaauuc
uagagcuaauacaugcuaaucucgacccuuuggaagagauguauuuauua
gaucaaugucuucggacucuuugaugaaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaaggca
gcaggccaaauuacccaauccuaauucagggagguagugacaauaaauaa
cgauacagggcccauucgggucuuguaauuggaaugaguacaauguaaau
accuuaacgaggaacaauuggagggcaagucuggugccagcagccgcggu
aauuccagcuccaauagcguauauuaaaguuguugcagaagcucguaguu
gaacuggcccgguuggccgguccggauuuccaacggggccuugauuaaua
gggacggucgggggcaucaguauucaauugucagaggugaaauucuugga
uuuauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauu
aaucaagaacgaccauaaacuaugccgacuagggaucgggugguguuuuu
uuaaugacccacucggcaccuuacgagaacuggggggaguauggucgcaa
ggcugaaacuuaaaggaauugacggaagggcaccaccaggaguggagccu
gcggcucaacacggggaaacucaccagguccagacacaauaaggauugac
auucuugauuuugugggugguggugcauugaugcccuuguucugggccac
gcgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucuu
gguaaucuugugaaacuccgucgugcuggggauauucaacgaggaauucc
uaguaagcgcaagucaucagcuugcguugauuacgucccugcccuuugua
cacaccgcccgucgcuaguaccgauugaauggcuuagugaggccucagga
ucugcuuagagaagggggcaacuccaucucagagcggagaauuuggacaa
acuuggucauuuagaggaacuaaaagucguaacaagguuuccguagguga
accugcggaa

...........................<<<<.<<<<<<...<....<<<.
<<...<<....<<....<<..........>>...>>.>>.....<<<...
....<<<..<<..<<....<<<..........<....<<<.<<<.....>
>>.>>.>....>.......<<<<............>>>>.....<<<<<.
..<<<<.<........>...>>>>...>>>>>.........<<<<..<<<
.....>>>.>>>>....>>>...>>>>..>>>.<.<....<<<....<<<
<<<<<.......>>>>>>>>>>>......>.>.....<<...........
....>>..>>>.<<.<<<..........>>>.>>...<<....>>.....
.>>>>>......<<<....<<<.....>>>..>>>.........>.....
<<<<<<<.<<<<<....>>>>>.>>>.>>>>......<<..<........
...>..>>.........<<<<<<.......<<<....>>>..........
........>>>>>>..>>>>>>>>>>..............<.<<...<<<
.<<..<<<<<.<<<<<..<.......>..>>>>>.>>>>>....>>....
<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>.....
.>.>>>>>>..>>.......<<....>>...>>>>..>.>>>>.>>>...
>>>...>>.>...............<<...<<<..<<<<<<<<.<<<...
.....>>>>>>>>>>>..>>>..>>.........................
..<<.........>>.....<<<<<<<<<<<<..<<.<<<<<<..<<<.<
<<<.......................<<......<<<<<...........
..........>>>>>......<<<........>>>.....>>.......>
>>>.>>>.....<<<<<<<............<<<..<<<<....>>>>..
>>>............>>>>>>>.......................>>>>>
>....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>....
........>>>>>..<<<<.<<.....<<<<<<<.<<<..<.<<......
<<<<<<<...<<.<<<<....>>>>.>>...>>>>>>>......>>.>..
>>>.>>>>>>>....>>.>>>>................<<<<<<<<<...
.>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6ke6 3.4 angstrom cryo-EM structure of yeast 90S small subunit preribosome
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): Q303 R306 R307 F849
• Binding residue (residue number reindexed from 1): Q53 R56 R57 F176

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding
• GO:0005524 ATP binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6ke6, PDBe:6ke6, PDBj:6ke6
• PDBsum: 6ke6
• UniProt: Q04500|UTP14_YEAST U3 small nucleolar RNA-associated protein 14 (Gene Name=UTP14)