Structure of PDB 6nuo Chain RG Binding Site BS02

Receptor Information

>6nuo Chain RG (length=181) Species: 300852 (Thermus thermophilus HB8)

PLDVALKRKYYEEVRPELIRRFGYQNVWEVPRLEKVVINQGLGEAKEDAR
ILEKAAQELALITGQKPAVTRAKKSISNFKLRKGMPIGLRVTLRRDRMWI
FLEKLLNVALPRIRDFRGLNPNSFDGRGNYNLGLREQLIFPEITYDMVDA
LRGMDIAVVTTAETDEEARALLELLGFPFRK

Ligand information

>6nuo Chain RA (length=2881)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
ugcgcggggggaaccuggggaacugaaacaucucaguacccagaggagag
gaaagagaaaucgacucccugaguagcggcgagcgaaaggggaccagccu
aaaccguccggcuuguccgggcggggucguggggcccucggacaccgaau
ccccagccuagccgaagcuguugggaagcagcgccagagagggugaaagc
cccguaggcgaaagguggggggauaggugaggguacccgaguaccccgug
guucguggagccaugggggaaucugggcggaccaccgccuaaggcuaagu
acuccgggugaccgauagcgcaccaguaccgugagggaaaggugaaaaga
accccgggaggggagugaaauagagccugaaaccgugggcuuacaagcag
ucacggccccgcaagggguuguggcgugccuauugaagcaugagccggcg
acucacggucgugggcgagcuuaagccguugaggcggaggcguagggaaa
ccgaguccgaacagggcgcaagcgcaaaguccgcggccguggacccgaaa
ccgggcgagcuagcccuggccagggugaagcuggggugagacccagugga
ggcccgaaccggugggggaugcaaaccccucggaugagcuggggcuagga
gugaaaagcuaaccgagcccggagauagcugguucuccccgaaaugacuu
uagggucagccucaggcgcugacuggggccuguagagcacugauagggcu
agggggcccaccagccuaccaaacccugucaaacuccgaagggucccagg
uggagccugggagugagggcgcgagcgauaacguccgcguccgagcgcgg
gaacaaccgagaccgccagcuaaggcccccaagucugggcuaagugguaa
aggauguggcgccgcgaagacagccaggagguggcuuagaagcagccauc
cuuuaaagagugcguaauagcucacuggucgaguggcgccgcgccgaaaa
ugaucggggcuuaagcccagcgccgaagcugcgggucugggggaugaccc
caggcgguaggggagcguucccgaugccgaugaaggccgacccgcgaggg
cggcuggagguaagggaagugcgaaugccggcaugaguaacgauaaagag
ggugagaaucccucucgccguaagcccaaggguuccuacgcaauggucgu
cagcguaggguuaggcgggaccuaaggugaagccgaaaggcguagccgaa
gggcagccgguuaauauuccggcccuucccgcaggugcgauggggggacg
cucuaggcuagggggaccggagccauggacgagcccggccagaagcgcag
ggugggagguaggcaaauccgccucccaacaagcucugcgugguggggaa
gcccguacgggugacaaccccccgaagccagggagccaagaaaagccucu
aagcacaaccugcgggaacccguaccgcaaaccgacacaggugggcgggu
gcaagagcacucaggcgcgcgggagaacccucgccaaggaacucugcaag
uuggccccguaacuucgggagaaggggugcucccuggggugaugagcccc
ggggagccgcagugaacaggcucuggcgacuguuuaccaaaaacacagcu
cucugcgaacucguaagaggagguauagggagcgacgcuugcccggugcc
ggaaggucaaggggaggggugcaagccccgaaccgaagccccggugaacg
gcggccguaacuauaacgguccuaagguagcgaaauuccuugucggguaa
guuccgaccugcacgaaaagcguaacgaccggagcgcugucucggcgagg
gacccggugaaauugaacuggccgugaagaugcggccuacccguggcagg
acgaaaagaccccguggagcuuuacugcagccugguguuggcucuugguc
gcgccugcguaggauaggugggagccugugaacccccgccuccggguggg
ggggaggcgccggugaaauaccacccuggcgcggcugggggccuaacccu
cggauggggggacagcgcuuggcgggcaguuugacuggggcggucgccuc
cuaaaagguaacggaggcgcccaaagguccccucaggcgggacggaaauc
cgccggagagcgcaaggguagaagggggccugacugcgaggccugcaagc
cgagcaggggcgaaagccgggccuagugaaccgguggucccguguggaag
ggccaucgaucaacggauaaaaguuaccccggggauaacaggcugaucuc
ccccgagcguccacagcggcggggagguuuggcaccucgaugucggcucg
ucgcauccuggggcugaagaaggucccaaggguugggcuguucgcccauu
aaagcggcacgcgagcuggguucagaacgucgugagacaguucggucucu
auccgccacgggcgcaggaggcuugaggggggcucuuccuaguacgagag
gaccggaagggacgcaccucugguuucccagcugucccuccaggggcaua
agcuggguagccaugugcggaagggauaaccgcugaaagcaucuaagcgg
gaagcccgccccaagaugaggccucccacggcgucaagccgguaaggacc
cgggaagaccacccgguggaugggccggggguguaagcgccgcgaggcgu
ugagccgaccggucccaaucguccgaggucu

.<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<<
<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>>
>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<<
.>>>>>>.>>...<<<<<<.....<......>......>>>>>.>.....
....<<....>>...<<<<<.....<<.....>>....>>>>>...<<<.
...<<<<<<<<.....>>>>>>>>......<<<<<<<<<....<<<...<
<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<.....))
>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<<<<<<<
<<.......>>>>>>>>>......<<<<<..((((>>>>>..>>>.....
.>>.>>>>>>.>>...............<<....>>....>>........
.<<<<....>>>>..................>>>>>>>>>>>.......<
<<<<<<<<<<....>>>>>>>>>>>...<.<........>.>.<<<<<<.
...<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<<....
>>..<<<<.....>>>>....<..>...>>>>>>>>>>>>>..<<.<..<
<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>>>>...
..>>>...<<<..<<<<........>>>>.>>>....>>>>>>>>>...<
<<.....>>>......>>>>>>...>..>.>>..<<<<<<....<<<<<.
...>>>>><<<<<<<<<.....<<<<<<<<<...<<<...<<<<<<<<..
.<<<<<<......>>>>.>>...>>>>>>>>..>>.>...>>>>>>>>>.
....>>>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>.....<<<
......>>>...<<<<.<<<...<<<(((.....<<<<<<..........
.<<..<<<<<<<<<........<<<<<.<<<..<<<.......>>>...>
>>.......<.<........>.>.>>>>>....>>>>>>>>>.>>.....
.....)))......>>>>>>.>>>...>>>>>>><<<<<<<<.....>>>
>>>>>....>>>>>><.<<<<<...<<<......<<<<<.<<<....>>>
>>>>>...>>>..>>>>>..>.....>>>>>><......<<.....<<<<
<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....
>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...
>>...<<<<<........>>>>>))<<<<<<<<<<<<<...<<<<....<
<<<<.<<<..<<<<<.<<<<.<<..[[[[...>>>>>><<<...<<<<<<
<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>>>>>>..>>><...
<<<<....>>>>.....>>>>>>...>>>..>>>>>.........>>>>.
..>>...>>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<
<<....>>>>>..>>>>..<<<<....<<<<<<<<.<<.........<..
...<<<<<.....<..>......>>>>><<<<<<<<<<<<.....>>>>>
>>>>>>>............<<<<<<<<<..<<<.........>>>..<<<
<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<
<<..<<<...<<...<<<<<....>>>>>...>>...>>>>>>>>..>>>
>><<<<<<.......>>>>>>........<...........<<<<<<...
..>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>>>>>>>
>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<.<<
<.........<<<<.<<<<<<..<<<<<.<<<.<<<<<.<<<<<<.<...
.<<<<..............<<...<.......<<<<<<<<<..>>>>>>>
>>....>.............>>.....>>>>....>.>>>>>>...<<<<
<....>>>>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<
<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>>
>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<......>>
>..>>>>.<<<....>>>.>>))).......<<<<<<<.<<..))))..>
>>>>>>>>.............>>>>..>>>>>>.......<<<<...<<<
<<<...<<.......>>...>>>>>>....>>>.>.......<<<<<.<<
<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>..
...>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.......
.>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<...........
....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<]]]]>>>>>>..
.>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>>>
....>>>.>>>>.......>>>>>>>...<<<.....>>>.....<<<<<
<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>
...>>>..>>>>>.>>>...>>>>....>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6nuo Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon-anticodon pairing.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): N40 G42 G44 E45 K47 T71 K74 N79 F80 I88 S124 D126 R128 N132 G134 L135 G154 M155
• Binding residue (residue number reindexed from 1): N39 G41 G43 E44 K46 T70 K73 N78 F79 I87 S123 D125 R127 N131 G133 L134 G153 M154

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6nuo, PDBe:6nuo, PDBj:6nuo
• PDBsum: 6nuo
• PubMed: 33016876
• UniProt: Q5SHQ0|RL5_THET8 Large ribosomal subunit protein uL5 (Gene Name=rplE)