Structure of PDB 5wfs Chain Q Binding Site BS02

Receptor Information

>5wfs Chain Q (length=115) Species: 562 (Escherichia coli)

ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRR
QRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVF
DKVAFTALVEKAKAA

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 5wfs Chain Q Residue 201

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5wfs Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): R5 I8
• Binding residue (residue number reindexed from 1): R5 I8
• Annotation score: 4

Gene Ontology

Molecular Function

• GO:0000900 mRNA regulatory element binding translation repressor activity
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5wfs, PDBe:5wfs, PDBj:5wfs
• PDBsum: 5wfs
• PubMed: 29733411
• UniProt: P0A7L3|RL20_ECOLI Large ribosomal subunit protein bL20 (Gene Name=rplT)