Structure of PDB 5obm Chain N3 Binding Site BS02

Receptor Information

>5obm Chain N3 (length=136) Species: 559292 (Saccharomyces cerevisiae S288C)

SGNGAQGTKFRISLGLPVGAIMNCADNSGARNLYIIAVKGSGSRLNRLPA
ASLGDMVMATVKKGKPELRKKVMPAIVVRQAKSWRRRDGVFLYFEDNAGV
IANPKGEMKGSAITGPVGKECADLWPRVASNSGVVV

Ligand information

>5obm Chain 2 (length=1712)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguucacaugguauaacugugguaa
uucuagagcuaauacaugcuuaaaaucucgacccuuuggaagagauguau
uuauuagauaaaaaaucaaugucuucggacucuuugaugauucauaauaa
cuuuucgaaucgcauggccuugugcuggcgaugguucauucaaauuucug
cccuaucaacuuucgaugguaggauaguggccuaccaugguuucaacggg
uaacggggaauaaggguucgauuccggagagggagccugagaaacggcua
ccacauccaaggaaggcagcaggcgcgcaaauuacccaauccuaauucag
ggagguagugacaauaaauaacgauacagggucuuguaauuggaaugagu
acaauguaaauaccuuaacgaggaacaauuggagggcaagucuggugcca
gcagccgcgguaauuccagcuccaauagcguauauuaaaguuguugcagu
uaaaaagcucguaguugaacuuugggcccgguuggccggucggauuucca
acggggccuuuccuucuggcuaccaggacuuuuacuuugaaaaaauuaga
guguucaaagcaggcguauugcucgaauauauuagcauggaauaauagaa
uaggacguuugguucuauuuuguugguuucuaggaccaucguaaugauua
auagggacggucgggggcaucaguauucaauugucagaggugaaauucuu
ggauuuauugaagacuaacuacugcgaaagcauuugccaaggacguuuuc
auuaaucaagaacgaaaguuaggggaucgaagaugaucagauaccgucgu
agucuuaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaa
ugacccacucggcaccuuacgagaaaucaaagucuuuggguucugggggg
aguauggucgcaaggcugaaacuuaaaggaauugacggaagggcaccacc
aggaguggagccugcggcuuaauuugacucaacacggggaaacucaccag
guccagacacaauaaggauugacagauugagagcucuuucuugauuuugu
gggugguggugcauggccguucuuaguugguggagugauuugucugcuua
auugcgauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauu
ugcugguuauccacuucuuagagggacuaucgguuucaagccgauggaag
uuugaggcaauaacaggucugugaugcccuuagacguucugggccgcacg
cgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucuug
guaaucuugugaaacuccgucgugcuggggauagagcauuguaauuauug
cucuucaacgaggaauuccuaguaagcgcaagucaucagcuugcguugau
uacgucccugcccuuuguacacaccgcccgucgcuaguaccgauugaaug
gcuuagugaggccucaggaucugccagagcggagaauuuggacaaacuug
gucauuuagaggaacuaaaagucguaacaagguuuccguaggugaaccug
cggaaggaucau

...<<<<<...[[[[>>>>>[[[[.((((((................[[[
.[[[..<<....<<....<<..........>>...>>.>>......{{..
......[[[..{{..{{....[[[...........<.....<<.<<....
...>>.>>......>.........<<<<<..<......>..>>>>>[...
((((((......<<<<<<<<<<<....>>>..............))))))
.....]...<<<<..<<<.....>>>.>>>>....]]]...}}}}..]]]
.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>......>>>
...<<<.<<<<....>>>>....>>>.}}.<<.<<<..........>>>.
>>.<.<<....>>.>...]]]]]].........<<<....<<<.....>>
>..>>>...............<<<<<..<....>..>>.>>>......<<
<.<...........>>.>>.........<<<<<((......<<<<.....
<<..))>>.......>>>>.>>>>>..))))))]]]].........[[[{
{.......{{...[[[.[[....<<<<<<.<<<<<....<..>....>>>
>>.>>>>>>....<<<<<<...>>>>>>...<<<<<.<<.......<<..
.<.......>..<<<.....>>>....>>......>>.>>..>>>.....
....[[...((((((((....>>>>>>>>.))))))))..]].....]].
...<<<<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>..
....>.>>>>>>..>>.......<<....>>...>>>>...>>>>>.>>>
...]]]...}}......<<<<<<<...<...<<<<.........>>>>..
.>>>>>>>>..........<<<.<<.<<<..<<<<<<<<.<<<.......
.>>>>>>>>>>>..>>>...<<..}}>>...>>.....>>>.]]].<<<.
.....<<<......>>>....>>>..]]]]..<<<<<.<<<<<<<..<<.
<<<<<<..<<<.<<<<<.......<<........>>..........<<<<
<......<<<<<........<<.<<<........>>>.>>......>>>>
>...<<.<<<..<<.<<<<<<....<<<.<<<<<....>>>...<<<...
...>>>...>>.>>>....<<<<<.<<..<<<<..<<<<<.<<<<<<...
.>>>>>>...>>>>>..>>>>.>>....<<<<<<.....>>>>>>.....
..>>>>>....>>>.>>>.....>>>>>>>.......>>>>>....>.>>
>>.>>>.....<<<<<<<......<<.....<<..<<<<....>>>>..>
>....>>.......>>>>>>>......<....<<<<<<..........>>
>>>>....>.....>>>>>>....<<<<<<<<.......>>>>>>>>...
...>>...>>>>>>>>>>.>>....<..<<.<..<<<<.<<....<<<<<
<<<.<<<..<.<<......<<<<......>>>>......>>.>..>>>.>
>>>>>>>...>>.>>>>...>.>>...>.....<<<<<<<<<....>>>>
>>>>>.......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5obm Aminoglycoside interactions and impacts on the eukaryotic ribosome.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): R32 P67
• Binding residue (residue number reindexed from 1): R31 P66

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5obm, PDBe:5obm, PDBj:5obm
• PDBsum: 5obm
• PubMed: 29208708
• UniProt: P0CX41|RL23A_YEAST Large ribosomal subunit protein uL14A (Gene Name=RPL23A)