Structure of PDB 8bn3 Chain N0 Binding Site BS02

Receptor Information

>8bn3 Chain N0 (length=172) Species: 4932 (Saccharomyces cerevisiae)

MAHFKEYQVIGRRLPTESVPEPKLFRMRIFASNEVIAKSRYWYFLQKLHK
VKKASGEIVSINQINEAHPTKVKNFGVWVRYDSRSGTHNMYKEIRDVSRV
AAVETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKF
PLPHRVQKSTKTFSYKRPSTFY

Ligand information

>8bn3 Chain 1 (length=3143)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucaggccagcaucaguuuu
gguggcaggauaaauccauaggaauguagcuugccucgguaaguauuaua
gccugugggaauacugccagcugggacugaggacugcgacguaagucaag
gaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaagg
agucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaauga
aagugaacguagguuggggccucgcaagaggugcacaaucgaccgauccu
gaugucuucggauggauuugaguaagagcauagcuguugggacccgaaag
auggugaacuaugccugaauagggugaagccagaggaaacucugguggag
gcucguagcgguucugacgugcaaaucgaucgucgaauuuggguauaggg
gcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguuuccc
ucaggauagcagaagcucguaucaguuuuaugagguaaagcgaaugauua
gagguuccggggucgaaaugaccuugaccuauucucaaacuuuaaauaug
uaagaaguccuuguuacuuaauugaacguggacauuugaaugaagagcuu
uuagugggccauuuuugguaagcagaacuggcgaugcgggaugaaccgaa
cguagaguuaaggugccggaauacacgcucaucagacaccacaaaaggug
uuaguucaucuagacagccggacgguggccauggaagucggaauccgcua
aggaguguguaacaacucaccggccgaaugaacuagcccugaaaauggau
ggcgcucaagcguguuaccuauacucuaccgucaggguugauaugaugcc
cugacgaguaggcaggcguggaggucagugacgaagccuagaccguaagg
ucgggucgaacggccucuagugcagaucuuggugguaguagcaaauauuc
aaaugagaacuuugaagacugaaguggggaaagguuccacgucaacagca
guuggacguggguuagucgauccuaagagauggggaagcuccguuucaaa
ggccugauuuuaugcaggccaccaucgaaagggaauccgguuaagauucc
ggaaccuggauauggauucuucacgguaacguaacugaauguggagacgu
cggcgcgagcccugggaggaguuaucuuuucuucuuaacagcuuaucacc
ccggaauugguuuauccggagauggggucuuauggcuggaagaggccagc
accuuugcuggcuccggugcgcuugugacggcccgugaaaauccacagga
aggaauaguuuucaugccaggucguacugauaaccgcagcaggucuccaa
ggugaacagccucuaguugauagaauaauguagauaagggaagucggcaa
aauagauccguaacuucgggauaaggauuggcucuaagggucgaucaacu
uagaacugguacggacaaggggaaucugacugucuaauuaaaacauagca
uugcgauggucagaaagugauguugacgcaaugugauuucugcccagugc
ucugaaugucaaagugaagaaauucaaccaagcgcggguaaacggcggga
guaacuaugacucucuuaagguagccaaaugccucgucaucuaauuagug
acgcgcaugaauggauuaacgagauucccacugucccuaucuacuaucua
gcgaaaccacagccaagggaacgggcuuggcagaaucagcggggaaagaa
gacccuguugagcuugacucuaguuugacauugugaagagacauuuauag
uuucuuuacuuauucaaugaagcggagcuggaauucauuuuccacguucu
agcauucaaggucccauucggggcugauccggguugaagacauugucagg
uggggaguuuggcuggggcggcacaucuguuaaacgauaacgcagauguc
cuaaggggggcucauggagaacagaaaucuccaguagaacaaaaggguaa
aagcccccuugauuuugauuuucagugugaauacaaaccaugaaagugug
gccuaucgauccuuuagucccucggaauuugaggcuagaggugccagaaa
aguuaccacagggauaacuggcuuguggcagucaagcguucauagcgaca
uugcuuuuugauucuucgaugucggcucuuccuaucauaccgaagcagaa
uucgguaagcguuggauuguucacccacuaauagggaacgugagcugggu
uuagaccgucgugagacagguuaguuuuacccuacugaugaauguuaccg
caauaguaauugaacuuaguacgagaggaacaguucauucggauaauugg
uuuuugcggcugucugaucaggcauugccgcgaagcuaccauccgcugga
uuauggcugaacgccucuaagucagaauccaugcuagaacgcggugauuu
cuuugcuccacacaauauagauggauacgaauaaggcguccuuguggcgu
cgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccuugg
gugcuugcuggcgaauugcaaugucauuuugcguggggauaaaucauuug
uauacgacuuagauguacaacgggguauuguaagcaguagaguagccuug
uuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.(.......<<<......)>>>..>.>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<..<<<<<<<.<<<<<<<
<<<<<<<<.....<<<<<<<<<......<<<<<......>>>>>......
.>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>...
..>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<<.
...<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...<<..
..>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<.
..<<....>>...>>>.>>>.>>....>>>>>>.>>>>>>..<<.<..<<
<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>....
.>>>...<<<..<<.<<........>>>>.>>>....>>>>>>>>>...<
<.......>>......>>>>>>...>..>.>>..<<<<<<....<.<<<.
...>>>.><<<<<<<<<<...<<<.<<<<<<<.....<<<....<<<..<
.<<<<...<<<<<<.....>>>>>>.>>>>.>..>>>..>.>>.....>>
>>>...<<<<.<<<<.<......>>>>>.>>>>.....>>>>>.>>>>>>
>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>..
.<<<<<<<..<<<<(((.....<<<<<<.....<<..........<<<.<
<<<<<<<<<.......<<<<<.......<<.........>>.........
...................>>>>>...>>>>>>>>>>>>>..........
))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<.........>>>
>>>>>>....>>>>>><.<<<<<<<<.<<......<<<<.<<<<....>>
>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<<
<<<.......>>>>>><<<<.....<<<<<.<<...<<<<<<...<<...
..>>.>>>>>>....((<<<<<<...<<....<<<....>>>........
<<<<<<........>>>>>>....>>....>>...<<<<<........>>
>>>))<<<<.<<<<<<<.....<<<....>>>.......<<<<<<...<<
<<.<<<<<<<.<<<<<<<<<<..[[[..>>>>>><...<<<<<....<<<
<<<....<<<.....>>>....>>>>>>.....>>>>>....><<<<<<<
<....>>>>>>>>>>>>...>>>>>>>.>>>>.........>>>>>>...
.......>>>..>>>>>>>>>>>>..>>.....<<......>>>>>>>.<
<<<.....>>>>..>>>>..<<<<.....<<<<<<<.<<.........<.
....<<<<<...............>>>>><<.<<<<<<<<<..>>>..>>
>>>>.>>............<<<<<<<<<..<<<.........>>>..<<<
<<<<....<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<
<<.<...<<.....<<<<....>>>>.....>>.>>>>>..>>>>><<<<
<<.......>>>>>>........<...........<<<<<.......>>>
>>.....>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>
>>....>>....<<<<<........>>>>>....<<<<<..<<<.<<...
...<<<<.<<<<<<..<<<<<.<<<<<<<<..<<<<<<<<<<.......>
>>>>>>>>>...<<<<<.....<<<<..<<<<........>>>>......
.........<<<<<<....>>>>>>..>>>>..>>>>>.....>>>>>>>
>..>>>>>...<<<.....>>>..<<<<<<...........>>>>>>..<
<...<<<<<<<...<<<<<<.......>>>>>>.....<<...(((>>..
..>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....>>>.>
>)))......<<<<<<<..<<<<.))))))>>>>>>>>>>>.........
>>>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>>...>
>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<......
>>>>>>>...>.<<<.........>>>.....>>>>>..>...>>>.>>.
...<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<<<<<<
<..<<<<...<<<<<<...............>>>>>>..<<<<<...<<<
<....<<<<<<<<<<]]].>>>>>..>>>>>...>>>>..>>>>>.<<<<
...<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>>..
.....<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>>
>......>>.......<<<<<<...<<<<<.<..<<<......>>>..>.
>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<...
..........>.....<<<<<<<<<.<<<....<<<<.<..<<<<<....
..>>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8bn3 Evolving precision: rRNA Expansion Segment 7S enhances translation accuracy in eukaryal ribosomes
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): M1 A2 N62 I64 N65 E66 H68 P69 K71 S83 R84 S85 H88 M90 Y91 K92 Q108 A111 A112 R113 H114 R115 R117 T132 R137 Y139 L144 T145 K146 H154 R155 K158 K161 Y165 P168 S169 T170 F171
• Binding residue (residue number reindexed from 1): M1 A2 N62 I64 N65 E66 H68 P69 K71 S83 R84 S85 H88 M90 Y91 K92 Q108 A111 A112 R113 H114 R115 R117 T132 R137 Y139 L144 T145 K146 H154 R155 K158 K161 Y165 P168 S169 T170 F171

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8bn3, PDBe:8bn3, PDBj:8bn3
• PDBsum: 8bn3
• PubMed: 38324474
• UniProt: P0CX23|RL20A_YEAST Large ribosomal subunit protein eL20A (Gene Name=RPL20A)