Structure of PDB 8brm Chain Li Binding Site BS02

Receptor Information

>8brm Chain Li (length=120) Species: 184922 (Giardia lamblia ATCC 50803)

PGLKAKDLVGLSQEDLQRKLADLKRELLSLRTMKATAGPERIARFRVCKK
DVARVLTVINQKARDEARAAFEGAEHIPKTFRPRLTHAMRCQLTEKQKRL
LPSKLMKRKLQFPKLKYAVK

Ligand information

>8brm Chain Lt (length=2594)

ggcgggccugaggcggcgggggcgacgggcggaacuuaagcauaucagua
cgccccggaggagaaaccaaccgggauuccccgcuagcggcgagcgacgc
gggaggagcccgccccgaaggcgcgcuguggggccgcgagggggcccgag
ggccccgcccgagagggugcaagccccguacggcgaguagcgcugcuuga
gcgugcagcgcgaagggaggcgcggcccuuccaaggcuaaauacgccccg
ggaccgauagcggaccaaguagcgcgagcgaacggugaaaaggacgcccu
gcggccgcucaaaagaccugaacccggccggccgccggcccgccggcccc
gucucgaaacacggaccgaggagccacgcgccgcggcgagcccgagggag
cccccgcggcggagcgagcgcgagacgccccgggcccgccaugccccugc
gggcgugcgcgggccgagccgcggcgcgugggcccgaaaggcggugaucu
augcccggcgagggcgaggccgggcgaaagccugguggaggcccgccgcg
gugcugacgcgcagaucgcucgucggagccgggcaugggggcgaaagacu
caucgaaccgccugguagcugguugccuccgaaaugucucccaggacagc
cgccgccccgcaguugcggcccguagagcgcuggccggcgggagcggggg
gccugccccucgcccgccccccaaacuccgaagggccgcgccgccccgcc
gcuggccugggcggggcgggcgaaugcgggcggcgcgugggccccuccug
guaagcaggacgggcgaggcgggacgauccggacgccgggccagggugcg
ccgccggggcccgcggaacggcgucggccggucccgacagccgaaucggc
cggcccggaaaauggagcgcgccggagccccggacccgcgcccggccgcc
gcgcgcggcggguaggaggccgcagaggccccgggggcgaaggcggcgcg
caggccccgccggaccggccucuggugcagaucucggcagcaguagccgc
uacuccgcgccccggaggacugagggggagacggguuccgcggcgccugc
aucuggccgcgggugacucgggccuaagcggcgggugaagaccgggaagg
ggcgugcccgcccgucgaacggggagccggcggagacuccggcaggcgcg
gcccccgcggagacgcccgccccccggcgacgcgcacggggaccgcggcg
ggcggcgccccggcccgcgaacgccccgcagcccccggacgccuugcgcg
gagaggggggcccgggggcggaccgcgcguccccggccgccccugaaaag
ccggggggcgccggccgcgcgccguaccgaccgcagcaggacuccggggu
cagcagccucuagcgcgggagcgaacgcggcucagggaagucggcaagcc
ggcuccguaaccucgggaaaaggaguggcucugacggcccgggucagaac
uggaacggacgcggggaucccgacuguuuacuagaaacacagcgucgcga
gggccgcacccggcgcuggcgcgacgugauuucugcccagugccacgacc
gucaccgugaagcgauccgccgaagcccugguaaacggcgggaguaacua
ugacucucuuaagguagccaaaugccucgucgggcaauuuccgacgugca
ugaauggaccaacgaggaucccacugucccgagccgcgccuccgcgagcc
uccagccucgggaacgggcgagggccggccagcggggcaagaagacccuu
uugagcuugacuccagcccgggccuguggggcggggcggccggcgcagcg
cacaggggaggccgcgccccugagacacccugacggccgccgccgccccg
cucacccggucgcgcggggacccgcccgggcggggaguucggcuggggcg
gcgcgccugcuacaccggaccgcaggcgucccacggcgggcucagcgagg
acggagaccucccgcggagcagaagggcacaagcccgcccgacccgcgcc
ccccgugccggcgcgggccgcgaaagcggggccuaccgauccuucgccgc
cccggccgcgggcgcggagguggcagaaaaguuaccacagggauaacugg
cuuguggccgccgagcgcccgcagcgacgcggcuuuuugauccuucgaug
ucggcucuuccuaccguccgcgcgcaccggcgcggaagcgucggauuguu
cacccguucaagggaucgugagcuggguuuagaccgucgugagacagguu
aguuuuacccuacuggccccggggccagagcacggcgggccaguacgaga
ggaacgcccgccgcgggccgccagccccgcgguugcccggccgggcagcg
ccgcgccgccgcgcccgggggcccugcgcugaccgccucuaagcgcgcac
cccgccucgcgccccgcccggccgcgcgccccagccccgugccccgucgc
cgagcggcccccgcccggggagaccacccggcgcggcgcuccuguacggc
gcagagcccugcgaucgccugagggacgcgccugcagagcgcgg

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.....................<<....>>................<<<..
..>>>............................<<<<<<..>>>>>>.<<
<<........>>>>.<<<<<....<<<<<<<<<<<<<..<<<...<<<..
..>>>..>>>...<<....>>............<<<<<<<<<<<<<....
>>>>>>>.>>>>>>...>>>>>>>>>>>>>..<<.<..<<<<<<<.....
<<<<<<<<<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<
..<<.<<........>>>>.>>>....>>>>>>>>>...<<.......>>
......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><<
<<<<<<<<.<<<.<<<<<<<<<...<<<...<<...<<<<<<...<<<<.
.......>>>>.>>>>>>...>>..>>.>...>>>>>>>.<<<<<<<<<<
.........>>>>>>>>>>...>>>>>>>>>>>>>.>>..<<<<.<<<<<
.....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((
...<<<<<<<........<<<.<<<<<<<<<..............>>>>>
>>>>>>>..........)))......>>>>>>>>>>>..>>>>>>><<<<
<....>>>>>....>>>>>><.<<<<<<<<...<<......<<<<<.<<.
...>>.>>>>>...>>>>>>>>>>..>.....>>>>>.<<.....<<...
..<<<<<.....>>>>>..<<.....<<<<..<<...<<<<<<...<...
....>.>>>>>>....((<<<.<<...<<<...<<<....>>>......<
<<<..>>>>...>>.>....>>...<<<<<........>>>>>))<<<<<
<<<...<<<....>>>..<<<<<<<<<<...<<<..<<<<<<<<<<<<<<
<<<...{{{...>>>>>....<<<<<<...<<<<<<....<<.....>>.
.....>>>>>>...>>>>>>..>>>>>.>>>>>>>..>>>.........>
>>>>>>>>>...>>>>>>>>.>>>..>>..<<......>>.>>>>.<<<<
.....>>>>..>>....<<<<....<<<<<<<<.<<.........<....
.<<<<<....<....>.....>>>>><<.<<<<<<<<.>>>..>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<....>>>>......>>>>>>>...<<..<<<..<<<<<<.<...
<<....<<<.<....>.>>>....>>.>>>>>..>>>>><<<<<<.....
..>>>>>>........<...........<<<<<<.....>>>>>>.....
>.....>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>....>
>....<<<<<........>>>>>...((((((..{{{.........{{{.
.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<.<<<..<<.........
..................................>>..>>>..>>>>>>>
>...<<<<......>>>>.....>>>>>>>>..>>>>>...<<<.....>
>>..<<<<<<...........>>>>>>..<<...<<<<<<<...<.<<<<
.......>>>>.>.....<<...(((>>....>>>>>>>...<<<<<<<<
.........>>>>>>>>.<<<....>>>.>>)))......((((((.(((
((.>>>>>>))))))))))).........]]]]..}}.}}}.......<<
.<...<<<<<<<..<<.......>>>..>>>>>>....>.>.>.......
<.<<<..<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<......
...>>>....>>>>>......>>>..>....<<<<<<<....>>>.>>>>
........}}}.))))))..<<<<<..<<<..<<<<<<<<..........
.....>>>>>>>><<<<<...<.<<...<<<<<<<<<<}}}.>>>>>..>
>>>>...>>.>.>>>>>.<<<...<<<<<<...........>>>>>>...
>>>..>>>..>>>>>....<<<<<<.........................
...>>>>>>...<<.<<<<.......>>>>..<<<<<<<<<<<....<<<
<....<<...>>...>>>>...>>>>.>>>>>.>>.....>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8brm Insights into translocation mechanism and ribosome evolution from cryo-EM structures of translocation intermediates of Giardia intestinalis.
• Resolution: 3.33 Å
• Binding residue (original residue number in PDB): H107 R113 H118 S134 K135 L136 R139
• Binding residue (residue number reindexed from 1): H76 R82 H87 S103 K104 L105 R108

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8brm, PDBe:8brm, PDBj:8brm
• PDBsum: 8brm
• PubMed: 36912103
• UniProt: A0A644FBK5