Structure of PDB 5wlc Chain LU Binding Site BS02

Receptor Information

>5wlc Chain LU (length=457) Species: 1247190 (Saccharomyces cerevisiae BY4741)

MKIKTIKRSADDYVPVKSTQESQMPRNLNPELHPFERAREYTKALNATKL
ERMFAKPFVGQLGYGHRDGVYAIAKNYGSLNKLATGSADGVIKYWNMSTR
EEFVSFKAHYGLVTGLCVTQPRFHDKKPDLKSQNFMLSCSDDKTVKLWSI
NVDDYSNKNNEEGLIRTFDGESAFQGIDSHRENSTFATGGAKIHLWDVNR
LKPVSDLSWGADNITSLKFNQNETDILASTGSDNSIVLYDLRTNSPTQKI
VQTMRTNAICWNPMEAFNFVTANEDHNAYYYDMRNLSRSLNVFKDHVSAV
MDVDFSPTGDEIVTGSYDKSIRIYKTNHGHSREIYHTKRMQHVFQVKYSM
DSKYIISGSDDGNVRLWRSKAWERSNVKTTREKNKLEYDEKLKERFRHMP
EIKRISRHRHVPQVIKKAQEIKNIELSSIKRREANERRTRKDMPYISERK
KQIVGTV

Ligand information

>5wlc Chain L1 (length=1025)

agauaguuaucugguugauccugccaguagucauaugcuugucucagcca
ugcagcucauuaaaucaguuaucguuuauuugauagucaugguauaacug
ugguaauucuagagcuaaaaucucgacccuuuggaagagauguauuuauu
agaucaaugucuucggacucuuugaugaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaagggc
aggcuuacccaauccuaauucagggagguagugacaauaaauaacgauac
agggcccauucgggucuuguaauuggaaugaguacaauguaaauaccuua
acgaggaacaauuggagggcaagucuggugccagcagccgcgguaauucc
agcuccaauagcguauauuagcucguaguugaacuauuaauagggacggu
cgggggcaucaguauucaauugucagaggugaaauucuuggauuuauuga
agacuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaaga
acgaaaguuaggggaucgaagaugaucagauaccgucguagucuuaagga
guauggucgcaaggcugaaacuuaaaggaauugacggaagggcaccacca
ggaguggagccugcggcucaacacggggaaacucaccagguccagacaca
auauuugugggugguggugcaugugaugcccuuguucuggcgcgcgcuac
acugacggagccagcgagucuaaccuuggccgagaggucuugguaaaaac
uccgucggggaacgaggaauuccuaguaagcgcaagucaucagcuugcgu
ugauuacgucccugcccuuuguacacaccgcccgucgcuaguaccggaac
uaauuccguaggugaaccugcggaa

.............................<<.<<<<<<........<<<.
<<<..<<<.......<<<..<<..<<....<<<..............<<.
<<<.....>>>.>>.....<<<<<..<<....>>..>>>>>....<<<<.
...<<<<.<<<....>>>...>>>>...>>>>.........<<<<..<<<
.....>>>.>>>>....>>>...>>>>..>>>.<<<....<<.....<<<
<<<<<.......>>>>>>>>.>>......>>>....<<<...<.......
.>..>>>.>>>.<<.<<<..........>>>.>>.<.<<....>>.>.>>
>>>>..<<<....<<<.....>>>..>>>...............<<<<<<
<.<<<<<....>>>>>.>>>.>>>>......<<..<..<....>...>..
>>.........<<<<<<.......<<<....>>>................
..>>>>>>..>>>>>>>>..<.<<...<<<.<<...>>....<<<<<<.<
<...<<<<..<<<.<<<<<<.<...<<<......>>>......>.>>>>>
>.>>>......<<<....>>>..>>>>..>>.>>>.>>>...>>>...>>
.>.....<<<<<<...<...<<<<.........>>>>...>>>>>>>...
..............<<.........>>.....<<<<<<<<<<<<..<<.<
<<<<<..<<<.<<<<....................<<<<<......<<<<
<...>>>>>......<<<<<<..>>.>>>>.....>>>>>>>>>.>>>..
...<<<<<<<............<<<..<<<<....>>>>..>>>.....>
>>>>>>............>>>>>>....<<<<<<<.........>>>>>>
>......>>...>>>>>>>............>>>>>..<<<<.<<>>.>>
>>.<<<<<<<<<....>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5wlc The complete structure of the small-subunit processome.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): R296 N299 V300 K302 G337 H338 R412 R415 H416 H418 V419 Q421 K438 Y453 R457
• Binding residue (residue number reindexed from 1): R288 N291 V292 K294 G329 H330 R404 R407 H408 H410 V411 Q413 K430 Y445 R449

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5wlc, PDBe:5wlc, PDBj:5wlc
• PDBsum: 5wlc
• PubMed: 28945246
• UniProt: P33750|DCA13_YEAST Protein SOF1 (Gene Name=SOF1)