Structure of PDB 8rxh Chain LM Binding Site BS02

Receptor Information

>8rxh Chain LM (length=203) Species: 347515 (Leishmania major strain Friedlin)

GAFMYLNELWKKKSSDVMRFIQRIRSWEFRHQHTVVRLRRPTRPEKARML
GYKTKQGFCVFRVRVRRGGRKRPVHKGITYGKPKTSGVLGMKLNKNNQAV
AEQRLGKRFGNLRVLNSYWVNMDSTFKWYEVIAVDPMCKTIRRDPRINWI
VNSVHKHREQRGLTSAGRKHRGLRHKGHKASKLRPSYRAAWRRNNRIVFL
RKR

Ligand information

>8rxh Chain L2 (length=1155)

cccaacugcagaccguacucaucaccgcaucagguccccaagcauagaau
gcucugguacauagagaaagguacacucagggaagucggcaaaauagaug
cguaaguucgcaagaagcauuggcucugagggcaaaaaauugccaacuca
gaacugcuuacggcggggaauccaacuguauaauuaaaacauagguuugu
gaugcauccaagugguguauaucgcaaacugauuucugcccagugcucug
aaugucaacgugacgagauucaccgacgcgcggguaaacggcgggaguaa
cuaugacucucuuaagguagccaaaugccucgucuuccaauuagagacgc
gcaugaauggauuaaugagauucccucugucccgaguuacuaucuagcga
aacgacagucaagggaacggacuugaagggcuaagcggggaaagaagacc
cuguugaguuugacuccagucuggcucugugcggcgacaucugaggcucg
gaacguugcuucacuuaucgaaugaagagaccaauggguuuagcguaguc
uucgggcuaugcaccgucuagguuugggaguccccuguggguacccaacc
gugcuguaaacugaaccaacgggauugcaguuccggagucuuguuucgag
acaucugccagauggggaguuuggcuggggcggcauaucuguuacacgac
aacgcagguguccuaaggcgagcucagugggaacagaaaucucacguaga
acacaagcguaaaagcuugcuugauuaacgauuuccaguacgaaucgaga
cugcgaaagcaaggccagcgauccuuugcgaagaauaggaaauaugaaca
aaucauaccagaggugucagaauaauuaccacagggauaacuggcuugug
gcggccaagcguucauagcgacgucgcuuuuugauccuucgaugucggcu
cuuccuaaccuagcgccgcagaagacgcuaaggguuggauuguucaccca
cugacagggaacgugagcuggguuuagaccgucgugagacagguugguuu
uacccuacuuagcuggaaauugcgagaaaaagauuauccgugcaaagcgg
acgucgggucguugggccuggcgagcgguggguaguacaugguacgcuca
ucacc

........................<<......>>......<<<<.....>
>>>........<<<<....<<<<.<<<<.<<.........<.....<<<<
<.....<..>......>>>>><<.<<<<<<<<<<<....>>>>>..>>>>
>>.>>..........<<<<<<<<<..<<<.........>>>..<<<<<<<
....<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<..
..<<<....<<<<......>>>>....>>>.>>>>..>>>>><<<<<<..
.....>>>>>>........<...........<<<<<.......>>>>>..
...>.....>>....>>.>>>>>>>..>..>>.>>>>>>>>.>>>>....
........<<<<<........>>>>>..<<<<<<<..<<<.<<......<
<<<.<<<<<<..<<<<<.<<<<<<<...<<<.<<<<<<.<<<.<<.>>.>
>>..>>>>>>.>>>...<<<<<.....<<<<....<<.....<<<<<<<<
....>>>>>>>>.>>.....<<<<.<<...<<.<..>.>>...>>.>>>>
...................<<<<<......>>>>>..>>>>...>>>>>.
......>>>>>>>..>>>>>...<<<.....>>>..<<<<<<........
...>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....
<<......>>....>>>>>>>...<<...<<<<<.......>>>>>..>>
.<<<....>>>.>>.........<<<<<..............<<<<....
...>>>>.>>>>>..........>>>>..>>>>>>.......<<.<.<.<
<<<<<...<<.......>>...>>>>>>...>>.>.>.......<<<<<.
<<<<<<..<<<<<<<.<......>.>>>>..>>>.<<<.........>>>
.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....>
>..>>>.>>>>>>>.......................<<<<.....>>>>
.<<<<<<<<<....>>>>>>>>>.<.<<<<<<<.<<<<...>>>>>>>>>
>>.>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8rxh Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.
• Resolution: 2.93 Å
• Binding residue (original residue number in PDB): R24 W28 R31 R67 G69 K72 P74 H76 K77 I79 T80 G82 S87 V89 L90 S125 T126
• Binding residue (residue number reindexed from 1): R23 W27 R30 R66 G68 K71 P73 H75 K76 I78 T79 G81 S86 V88 L89 S124 T125

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8rxh, PDBe:8rxh, PDBj:8rxh
• PDBsum: 8rxh
• PubMed: 38722744
• UniProt: Q4Q6S3