Structure of PDB 8fkz Chain LH Binding Site BS02

Receptor Information

>8fkz Chain LH (length=143) Species: 9606 (Homo sapiens)

KAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRK
SAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAV
KKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII

Ligand information

>8fkz Chain L3 (length=2833)

gcgaccucagaucagacguggcgacccgcugaauuuaagcauauuaguca
gcggaggagaagaaacuaaccaggauucccucaguaacggcgagugaaca
gggaagagcccagcgccgaauccccgccccggggcgcgggacauguggcg
uacggaagacccgcuccccggcgccgcgggggcccaaguccuucugaucg
aggcccagcccguggacggugugaggccgguagcggcccccggcgcgccg
ggcccgggucuucccggagucggguugcuugggaaugcagcccaaagcgg
gugguaaacuccaucuaaggcuaaauaccggcacgagaccgauagucaac
aaguaccguaagggaaaguugaaaagaacuuugaagagagaguucaagag
ggcgugaaaccguuaagagguaaacgggugggguccgcgcaguccgcccg
gaggauucaacccggcggcggccggccgugucggcggcccggcggaucug
ggaccgucccccgaccggcgaccggccgccgccgggcgcauuuccaccgc
ggcggugcgccgcgaccggcuccgggacggcugggaaggcccggcgggga
agguggcucgggcccgaguguuacagcccccccggcagcagcacucgccg
aaucccggggccgagggagucgccgcgcucuccccccggggggccgggcc
accccucccacggcgcgcgccgucgggcccggggggccacgcgcgcgucc
cccgaagagggggacggcggagcgagcgcacggggucggcggcgacgucg
gcuacccacccgacccgucaaacacggaccaaggagucuaacacgugcgc
gagucgggggcucgcacgaaagccgccguggcgcaaugaaggugaaggcc
gcggccgaggugggaucccgaggccgccgagggcgcaccaccggcccguc
ucgcccgccgcgccggggagguggagcacgagcgcacguguuaggacccg
aaagauggugaacuaugccugggcagggcgaagccagaggaaacucuggu
ggagguccguagcguccgaccuggguauaccaucuaguagcugguucccu
ccgaaguuucccucaggauagcuggcgcucuccgcaguuuuauccggggg
uaagaagcccggcucgcuggcguggagccgggcguggaaugcgagugccu
agugggccacuuuugguaagcagaacuggcgcugcgggaugaaccgaacg
ccggguuaaggcgcccgaugccgacgcucaucagaccagaaaagguguug
guugauauagacagcaggacgguggccauggaagucggaauccgcuaagg
aguguguaacaacucaccugccgaaucaacuagcccugaaaauggauggc
gcuggagcgucgggcccauacccggccgucgccggcagugacgcuacgcc
gcgacgaguaggagggccgcugcggugagccuugaagccuagggcgcggg
cccggguggagccgccgcaggugcagaucuuggugguaguagcaaauauu
caaacgagaacuuugaaggccgaaguggagaaggguuccaugugaacagc
aguugaacaugggucagucgguccugagagaugggcgagcgccguuccga
agggacgggcgauggccuccggcccucgaucgaaagggagucggguucag
auccccgaauccggaguggcggagaugggcgccgcgaggcguccagugcg
guaacgcgaccgaucccggagaagccggcgggagccccggggagaguucu
cuuuucuuugugaagggcagggcgcccuggaauggguucgccccgagaga
ggggcccgugccuuggaaagcgucgcgguuccggcggcguccggugagcu
cucgcuggcccuugaaaauccgggggagaggguguaaaucucgcgccggg
ccguacccauauccgcagcaggucuccaaggugaacagccucuggcaugu
uggaacaauguagguaaggggcucuaagggcugggucggucgggcugggg
cgcgaagcggggucccguggaucgccccagccggccggcgccuagcagcc
gacuuagaacugcaucgcgaaggcccgggguguugacgcgauguuguccc
uaccuacuauccagcgaaaccacagccaagggaacgggcuuggcggaauc
agacccggugaggcggggggcuucuggcgccaagcgcccggccgcgcgcc
ggccgggcgcgacccgcuccggggcuucugaccuuuuggguuuuaagcag
gagggcuuguggcggccaagcgagcgacgucgcuuuuugcggcucuuccu
aucauugugaagcagaauucaccaagcguuggauuguucacccacuaaua
gggaacgugagcuggcuacugaugauguguuguugccaugguaauccugc
ucaguacgagaggaaccgcagguucagacauuugguguaugugcuuggcu
gaggagccaauggggcgaagcuaccaucugugggauuaugacugaacgcc
ucuaagucagaaucccgcccaggcggaacgauagcgccgcggagccucgg
uuggccucggauagccgguccccgggguccggugcggagugcccuucguc
cugggaaacggggcgcggccggagaggcggccgcccccucgcccgucacg
caccgcacguucguggggaaccuggcgcuaaaccauucguagacgaccug
cuucugggucgggguuucguacguagcagagcagcucccucgcugcgauc
uauugaaagucagcccucgacacaaggguuugu

........................<<<<<<.....<<....>>......>
>>>>.>.........<<....>>..<<<<<<.....<<.....>>....>
>>>>>..[[[..<<<<<...<<<<<<<<<..>>>>>.>>>>.....>>>>
>..<<<<<<<<<<...<<<<<<<<.<<<<<<<<<<....<<<<......>
>>>.....((......<<<.....))>>>...>.>>>>>>>.>>>>>>>>
>>..>>>>>>>>>>.......<<<<<<<........>>>>>>>.....((
(((..<<<<<<)))))..]]].............................
.....<<....>>...............<<<......>>>..........
........................<<<<<<<<..<<<.<<...<<<<<<<
<.<<<.....<<<<<<<<<<<<<<<<<<.<<<<<.<<...<<<<<.<<<.
>>>>>>>>>>>>>>>>>>>...>>>>>>>>>>>>>>......>>>.>>>.
>>>>>...<<<<<<<<<<<<<<<<<<<<<<<...<<..<<<.<<.<<<<.
.....<<<<<<..>>>>>>.......>>>>>>.>>>........>>>>>>
..>>>>>>>>>>>...........<<<<<<.<<<<<..>>>>>.<<<<<<
.<<<<.<<<<<<<<<..>>>>>>.>>>...>>>>>>>>.>>..<<<<<<<
<<.......>>>>>>>.>>....>>>>>>.....>>>.>>>>>...>>>>
>...>>>>>>>>..<<<<.....>>>><<<<<<....<<<<<<<<<<<<<
..<<<...<<<<........>>>>.....>>>...<<....>>...<<<<
..>>>>..<<<<<...<<<..<<<.>>>..>>>....>>>>>.<<<<<<<
<<.<<<<......>>>>>>>>>>>.>>....>>>>>>>>>>>>>..<<.<
...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>
.....>>>...<..>....>>>>>>>>>>>>>>>......>.>>..<<<<
<<......<<<....>>>..<<.<<<<<<<...<<<.<<<<<<<<..>>>
>>>...<<<<<<<<<.<......>>>>>>>>>>....>>>>>>>>>>>>.
.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<......>>>...<
<<<<<<...<<<(((.......<<<<<.....<<........<<<.<<<<
<<<<<<.......<<<<<.<<<...<<.........>>...>>>......
...<<......>>...>>>>>...>>>>>>>>>>>>>..........)))
.>>...>>>>>..>>>...>>>>>>><<<<<<.<<<...........>>>
>>>>>>....>>>>>>.<.<<<<<<<<.<<......<<<<.<<<<....>
>>>>>>>...>>>>>>>>>>..>.....>>>>>><<.....<<.....<<
<<<<.......>>>>>>.<<<.....<<<<<.<<...<<<<<<...<<..
...>>.>>>>>>....((<<<<<<...<<....<<<....>>>.......
......<<<<...........>>>>....>>....>>>..<<<<<.....
...>>>>>))<<<<....<<<<<..<<<<<<<<....>>>>>>>>..<<<
....>>>...<..<<<<<<<...<<<<<<<<<<<<.<<<<<<<<<<..{{
{..>>>>>>....<<<<<.<<<<.<<<<.....<<<.....>>>.....>
>>>>>>>..>>>>>.....<<<<<<<.......>>>>>>>>>>>...>>>
>>>>>>>>>.........>>>>>>>..>..........>>.>>>.>>>>.
>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>....
<<<<.....<<<<<<<.<<.<.<<<<<<<<<<<.<<<<<<<<.<<<<<<<
<<....<<<<<..>>>>>....>>>>>>>>>>>>>>>>>......>>>>>
..>>>>>>.>.<<<<<<<....<<<<.>>>>......>>>>>>>...>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....[[[
[[.<<<<<.....<<<<................<<<<<<<<<<.......
>>>>>>>>>>..>>>>..>>>>>.((((((..(((..>>>>>>.))))))
))).......<<<<<<...<<..>>...>>>>>>.....<<<<.<<<<<<
..<.<.<<<<<......>>>>>...>.>.<<<.........>>>.....>
>>>>..>...>>>>....]]]]].....<<<<<<.<..<<<..<.<<<<<
.................>>>>>.><<<<....<<<<....<<.<<<<<<.
}}}..>>>>..>>.>>...>>>>...>>>><<<<<...<<<<<.......
....>>>>>...>>>>>.>>>...>.>>>>>>.<<<<<<.<<..<<<<..
..<<<........<<<<<.<<<<>>>>.>>>>>.......>>>...<<<.
.<<<<....<<<<.<<<<<<<......>>>>>>>>>>>...>>>>..>>>
..............>>>>..>>>>>>>>.<<<<<.<.............>
....<<.<<<<<<<.<<.....<<<<....<<<<<......>>>>>...>
>>>....>>....>>>.>>>>.>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fkz Principles of human pre-60 S biogenesis.
• Resolution: 3.04 Å
• Binding residue (original residue number in PDB): K37 P44 T45 F46 R48 K50 T51 R53 L54 R55 R56 Q57 K59 Y60 R62 T83 N105 K106 H107 N125 L127 I128 P130 K134 K135 R139
• Binding residue (residue number reindexed from 1): K24 P31 T32 F33 R35 K37 T38 R40 L41 R42 R43 Q44 K46 Y47 R49 T70 N92 K93 H94 N112 L114 I115 P117 K121 K122 R126

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0045296 cadherin binding
• GO:1904841 TORC2 complex binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fkz, PDBe:8fkz, PDBj:8fkz
• PDBsum: 8fkz
• PubMed: 37410842
• UniProt: P62750|RL23A_HUMAN Large ribosomal subunit protein uL23 (Gene Name=RPL23A)